12-Deoxyphorbol-13-isobutyrate-20-acetate

Cat. No.: HY-N18070: Data Sheet Handling Instructions
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12-Deoxyphorbol-13-isobutyrate-20-acetate is a 12-deoxy-phorbol diterpene ester. 12-Deoxyphorbol-13-isobutyrate-20-acetate is a protein kinase C (PKC) agonist that activates protein kinase C to phosphorylate troponin I and troponin T. 12-Deoxyphorbol-13-isobutyrate-20-acetate induces skin irritation and necrosis in mice without cocarcinogenic activity. 12-Deoxyphorbol-13-isobutyrate-20-acetate inhibits Aspergillus carbonarius growth. 12-Deoxyphorbol-13-isobutyrate-20-acetate supports research on heart failure, cardiomyopathies and fungal infection.

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12-Deoxyphorbol-13-isobutyrate-20-acetate 화학구조

CAS No. : 25090-71-5

사이즈		재고
MOQ		Minimum order quantity
50 mg		견적 받기
100 mg		견적 받기
250 mg		견적 받기
Other size		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target^[1]

PKC

In Vitro

12-Deoxyphorbol-13-isobutyrate-20-acetate (1 μM) reduces contractile force in intact human trabeculae carneae via calcium-independent mechanisms, and its negative inotropy and lowered myofilament calcium sensitivity are partially reversed by caffeine^[1].
12-Deoxyphorbol-13-isobutyrate-20-acetate (1 μM) decreases myofilament calcium sensitivity in skinned human trabeculae carneae (shifting [Ca²⁺]₅₀_% to 2.09 μM and reducing maximal force), while lowering contractile force and altering Hill coefficients in intact control and myopathic human trabeculae carneae with consistent [Ca²⁺]₅₀_% elevations^[1].
12-Deoxyphorbol-13-isobutyrate-20-acetate (10-100 μM; 3 days) decreases Aspergillus carbonarius growth by 25%^[3].
12-Deoxyphorbol-13-isobutyrate-20-acetate (0.001-100 μM; 72 h) reduces viability of both MCF7 breast cancer cells and MCF10A normal breast cells to a similar extent, with no selective cytotoxicity toward cancer cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[3]

Cell Line:	MCF7 breast cancer cells, MCF10A normal breast cells
Concentration:	0.001 μM, 1 μM, 10 μM, 100 μM
Incubation Time:	72 h
Result:	Reduced viability of MCF7 breast cancer cells and MCF10A normal breast cells to a similar extent, with no significant difference in cytotoxicity between the two cell lines at any tested concentration.

In Vivo

12-Deoxyphorbol-13-isobutyrate-20-acetate (0.024 nmoles per ear; topical; single dose) has an ID₅₀ of 0.024 nmoles per ear for inducing reddening in the mouse ear topical irritation model^[2].
12-Deoxyphorbol-13-isobutyrate-20-acetate (0.2 μmoles per dose; topical; twice weekly; 24 weeks) exhibits no detectable cocarcinogenic tumor-promoting activity in NMRI mice initiated with 7,12-dimethylbenz[a]anthracene, resulting in 0% tumor rate and 0 tumor yield over 24 weeks, with 28% mortality^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NMRI (female)^[2]
Dosage:	0.2 μmoles per dose
Administration:	topical; twice weekly; 24 weeks
Result:	Resulted in 0% of mice developing tumors at 12 weeks and 24 weeks. Achieved a tumor yield of 0 tumors per survivor at 12 weeks and 24 weeks. Caused 28% mortality after 24 weeks. Induced pronounced skin necrosis in the treated area after 2-3 applications.

분자량

460.56

화학식

C₂₆H₃₆O₇

CAS No.

25090-71-5

SMILES

CC(C)C(O[C@@]12[C@@]([C@@]3([H])[C@]([C@@H](C2)C)([C@@]4([H])[C@](CC(COC(C)=O)=C3)(C(C(C)=C4)=O)O)O)([H])C1(C)C)=O

Structure Classification

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Gwathmey JK, et al. Effect of protein kinase C activation on sarcoplasmic reticulum function and apparent myofibrillar Ca2+ sensitivity in intact and skinned muscles from normal and diseased human myocardium. Circ Res. 1990;67(3):744-752. [Content Brief]

[2]. Hergenhahn M, et al. Diterpene esters from 'Euphorbium' and their irritant and cocarcinogenic activity. Experientia. 1974 Dec 15;30(12):1438-40. [Content Brief]

[3]. Ourhzif EM, et al. Antifungal and Cytotoxic Activity of Diterpenes and Bisnorsesquiterpenoides from the Latex of Euphorbia resinifera Berg. Molecules. 2022 Aug 16;27(16):5234. [Content Brief]