2',7'-Dichlorofluorescein
Based on 1 Customer Validation
2',7'-Dichlorofluorescein is an important fluorescent product in the detection of oxidative stress and can be used to measure reactive oxygen species (ROS) (Ex=503 nm and Em=523 nm).
For research use only. We do not sell to patients.
- Purity: 99.22%
- CAS No.: 76-54-0
- Formula: C20H10Cl2O5
- Molecular Weight:401.20
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
The precursor of 2',7'-Dichlorofluorescein, 2',7'-Dichlorodihydrofluorescein diacetate, is lipophilic and can freely cross the cell membrane. After entering the cell, under the action of intracellular esterases, 2',7'-Dichlorodihydrofluorescein diacetate is converted into 2′,7′-Dichlorodihydrofluorescein (HY-153006). 2′,7′-Dichlorodihydrofluorescein is somewhat hydrophilic and remains inside the cell. When oxidants are present in the cell, it is oxidized to generate 2',7'-Dichlorofluorescein[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
525
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Chemical Information
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CAS No. 76-54-0
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Appearance Solid
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Molecular Weight 401.20
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Formula C20H10Cl2O5
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Color Yellow to orange
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(Cl)C(O)=C4)C=C(O)C(Cl)=C3)C5=C1C=CC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL (311.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Reiniers MJ, et al. Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89(7):3853-3857. [Content Brief]
[2]. Xiuping Chen, et al. 2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy. Free Radic Res. 2010 Jun;44(6):587-604. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4925 mL | 12.4626 mL | 24.9252 mL | 62.3131 mL |
| 5 mM | 0.4985 mL | 2.4925 mL | 4.9850 mL | 12.4626 mL | |
| 10 mM | 0.2493 mL | 1.2463 mL | 2.4925 mL | 6.2313 mL | |
| 15 mM | 0.1662 mL | 0.8308 mL | 1.6617 mL | 4.1542 mL | |
| 20 mM | 0.1246 mL | 0.6231 mL | 1.2463 mL | 3.1157 mL | |
| 25 mM | 0.0997 mL | 0.4985 mL | 0.9970 mL | 2.4925 mL | |
| 30 mM | 0.0831 mL | 0.4154 mL | 0.8308 mL | 2.0771 mL | |
| 40 mM | 0.0623 mL | 0.3116 mL | 0.6231 mL | 1.5578 mL | |
| 50 mM | 0.0499 mL | 0.2493 mL | 0.4985 mL | 1.2463 mL | |
| 60 mM | 0.0415 mL | 0.2077 mL | 0.4154 mL | 1.0386 mL | |
| 80 mM | 0.0312 mL | 0.1558 mL | 0.3116 mL | 0.7789 mL | |
| 100 mM | 0.0249 mL | 0.1246 mL | 0.2493 mL | 0.6231 mL |
2',7'-Dichlorofluorescein Related Classifications
HY-W040143 Related Classifications
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