2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. 2',7'-Dichlorofluorescein

2',7'-Dichlorofluorescein acts as a fluorescent probe (Ex=496 nm and Em=525 nm) for reactive oxygen species (ROS) measurement.

For research use only. We do not sell to patients.

2',7'-Dichlorofluorescein Chemical Structure

2',7'-Dichlorofluorescein Chemical Structure

CAS No. : 76-54-0

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Based on 1 publication(s) in Google Scholar

2',7'-Dichlorofluorescein Related Antibodies

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Description

2',7'-Dichlorofluorescein acts as a fluorescent probe (Ex=496 nm and Em=525 nm) for reactive oxygen species (ROS) measurement[1].

Molecular Weight

401.20

Formula

C20H10Cl2O5
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

525
Excitation (Ex)

496
SMILES

O=C1OC2(C3=C(OC4=C2C=C(Cl)C(O)=C4)C=C(O)C(Cl)=C3)C5=C1C=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (311.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4925 mL 12.4626 mL 24.9252 mL
5 mM 0.4985 mL 2.4925 mL 4.9850 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4925 mL 12.4626 mL 24.9252 mL 62.3131 mL
5 mM 0.4985 mL 2.4925 mL 4.9850 mL 12.4626 mL
10 mM 0.2493 mL 1.2463 mL 2.4925 mL 6.2313 mL
15 mM 0.1662 mL 0.8308 mL 1.6617 mL 4.1542 mL
20 mM 0.1246 mL 0.6231 mL 1.2463 mL 3.1157 mL
25 mM 0.0997 mL 0.4985 mL 0.9970 mL 2.4925 mL
30 mM 0.0831 mL 0.4154 mL 0.8308 mL 2.0771 mL
40 mM 0.0623 mL 0.3116 mL 0.6231 mL 1.5578 mL
50 mM 0.0499 mL 0.2493 mL 0.4985 mL 1.2463 mL
60 mM 0.0415 mL 0.2077 mL 0.4154 mL 1.0386 mL
80 mM 0.0312 mL 0.1558 mL 0.3116 mL 0.7789 mL
100 mM 0.0249 mL 0.1246 mL 0.2493 mL 0.6231 mL
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2',7'-Dichlorofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2',7'-Dichlorofluorescein76-54-0Reactive Oxygen Speciesfluorescent probereactive oxygen speciesROSInhibitorinhibitorinhibit

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