2-Bromo-6-nitrophenol
Based on 1 Customer Validation
2-Bromo-6-nitrophenol is a 2,6-disubstituted phenol. 2-Bromo-6-nitrophenol exhibits a unique pattern of plant growth regulatory activity: it shows high auxin activity in the pea segment, pea curvature, and tomato leaf epinasty assays, but displays very low or no activity in the wheat cylinder assay.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 13073-25-1
- Formula: C6H4BrNO3
- Molecular Weight:218.01
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
2-Bromo-6-nitrophenol undergoes rapid para-hydroxylation in wheat coleoptile segments to form inactive 2-bromo-6-nitroquinol, while this hydroxylation reaction rarely occurs in pea stem segments and tomato stem segments. Long-term incubation in wheat coleoptiles and pea stem segments leads to nitro reduction to produce 2-bromo-6-aminophenol, which is subsequently acetylated to form N-acetyl-2-bromo-6-aminophenol[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 13073-25-1
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Appearance Solid
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Molecular Weight 218.01
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Formula C6H4BrNO3
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Color Light brown to yellow
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SMILES
C1=C([N+]([O-])=O)C(=C(Br)C=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (458.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.5869 mL | 22.9347 mL | 45.8695 mL | 114.6736 mL |
| 5 mM | 0.9174 mL | 4.5869 mL | 9.1739 mL | 22.9347 mL | |
| 10 mM | 0.4587 mL | 2.2935 mL | 4.5869 mL | 11.4674 mL | |
| 15 mM | 0.3058 mL | 1.5290 mL | 3.0580 mL | 7.6449 mL | |
| 20 mM | 0.2293 mL | 1.1467 mL | 2.2935 mL | 5.7337 mL | |
| 25 mM | 0.1835 mL | 0.9174 mL | 1.8348 mL | 4.5869 mL | |
| 30 mM | 0.1529 mL | 0.7645 mL | 1.5290 mL | 3.8225 mL | |
| 40 mM | 0.1147 mL | 0.5734 mL | 1.1467 mL | 2.8668 mL | |
| 50 mM | 0.0917 mL | 0.4587 mL | 0.9174 mL | 2.2935 mL | |
| 60 mM | 0.0764 mL | 0.3822 mL | 0.7645 mL | 1.9112 mL | |
| 80 mM | 0.0573 mL | 0.2867 mL | 0.5734 mL | 1.4334 mL | |
| 100 mM | 0.0459 mL | 0.2293 mL | 0.4587 mL | 1.1467 mL |
2-Bromo-6-nitrophenol Related Classifications
HY-W040886 Related Classifications
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