3-Hydroxykynurenine
Based on 2 publication(s) in Google Scholar
3-Hydroxykynurenine, a metabolite of tryptophan, is a potential endogenous neurotoxin whose increased levels have been described in several neurodegenerative disorders. 3-Hydroxykynurenine induces neuronal apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 484-78-6
- Formula: C10H12N2O4
- Molecular Weight:224.21
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Apoptosis Compound Library
HY-L003
-
Immunology/Inflammation Compound Library
HY-L007
-
Metabolism/Protease Compound Library
HY-L012
-
Natural Product Library
HY-L021
-
Human Endogenous Metabolite Compound Library
HY-L030
-
Phenols Library
HY-L057
-
Food-Sourced Compound Library
HY-L094
-
Human Metabolite Library
HY-L123
-
Animal-Sourced Natural Product Library
HY-L157
-
Highly Selective Activators Library
HY-L159
-
Cell Death Library
HY-L162
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Bioactive Compound Library
HY-L001
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Mass Spectrometry Human Endogenous Metabolite Library
HY-L207
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Biotoxin Library
HY-L220
-
Mass Spectrometry Natural Product Library
HY-L262
Publications Citing Use of MedChemExpress (MCE) 3-Hydroxykynurenine
More
Customer Validation & Images
-
Cell Imaging/Staining
-
Flow Cytometry
-
Cell Proliferation/Viability Assay
-
Flow Cytometry
-
WB
All Endogenous Metabolite Isoforms
More
Biological Activity
|
Human Endogenous Metabolite |
3-Hydroxykynurenine-induced neuronal cell death shows several features of apoptosis[1].
3-Hydroxykynurenine is a metabolic intermediate of the kynurenine pathway, the major metabolic pathway of tryptophan (Trp)[1].
3-Hydroxykynurenine acts as an endogenous neurotoxin in the brain under pathological conditions[1].
3-Hydroxykynurenine (20-100 μM) significantly inhibits CD4+ T-cell proliferation in a dose-dependent manner with IC50 of approximately 70 μM[2].
3-Hydroxykynurenine (20-100 μM) induces significant CD4+ T-cell-mediated cell death in a dose-dependent manner[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:CD3/CD28 bead-stimulated CD4+ T cells
-
Concentration:0, 20, 40, 60, 80, and 100 μM
-
Incubation Time:72 hours
-
Result:Significantly inhibited CD4+ T-cell proliferation in a dose-dependent manner with an IC50 of approximately 70 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:BALB/c (H2d) mice[2]
-
Dosage:560 mg/kg
-
Administration:IP injection
-
Result:There was significant prolongation of graft survival in all treatment groups in comparison to the controls.
Chemical Information
-
CAS No. 484-78-6
-
Appearance Solid
-
Molecular Weight 224.21
-
Formula C10H12N2O4
-
Color Light yellow to yellow
-
SMILES
O=C(O)C(N)CC(C1=CC=CC(O)=C1N)=O
-
Synonyms
3-Hydroxy-DL-kynurenine
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
Int Immunopharmacol
Mechanism of Trichinella spiralis-mediated CD4+ T cell functional imbalance via IDO-dependent tryptophan metabolism. [Abstract]2026 May 15:177:116517. PMID: 41864014 -
Psychoneuroendocrinology
Peripheral cytokine dysregulation, microglial dysfunction in adolescent major depressive disorder: Neuroimmune crosstalk implications. [Abstract]2025 Oct 17:183:107656. PMID: 41138684
3-Hydroxykynurenine purchased from MedChemExpress. Usage Cited in: Psychoneuroendocrinology. 2025 Oct 17:183:107656. [Abstract]
After HMC3 cells were treated with indicated concentrations of 3-Hydroxykynurenine (3-HK, 10-160 μM), morphological changes were observed using an optical microscope.
3-Hydroxykynurenine purchased from MedChemExpress. Usage Cited in: Psychoneuroendocrinology. 2025 Oct 17:183:107656. [Abstract]
After HMC3 cells were treated with indicated concentrations of 3-Hydroxykynurenine (3-HK, 10-160 μM), Annexin V/PI staining was performed followed by flow cytometric analysis.
3-Hydroxykynurenine purchased from MedChemExpress. Usage Cited in: Psychoneuroendocrinology. 2025 Oct 17:183:107656. [Abstract]
After HMC3 cells were treated with indicated concentrations of 3-Hydroxykynurenine (3-HK, 10-160 μM), cell viability was analyzed by CCK-8 staining.
3-Hydroxykynurenine purchased from MedChemExpress. Usage Cited in: Psychoneuroendocrinology. 2025 Oct 17:183:107656. [Abstract]
After HMC3 cells were treated with indicated concentrations of 3-Hydroxykynurenine (3-HK, 10-160 μM), Sub-G1 analysis was performed by flow cytometry.
3-Hydroxykynurenine purchased from MedChemExpress. Usage Cited in: Psychoneuroendocrinology. 2025 Oct 17:183:107656. [Abstract]
After HMC3 cells were treated with indicated concentrations of 3-Hydroxykynurenine (3-HK, 40-160 μM), immunoblotting was performed for the indicated antigens.
3-Hydroxykynurenine purchased from MedChemExpress. Usage Cited in: Psychoneuroendocrinology. 2025 Oct 17:183:107656. [Abstract]
After HMC3 cells were treated with indicated concentrations of 3-Hydroxykynurenine (3-HK, 40-160 μM), culture supernatants were collected and the release of LDH and IL-1β was assessed.
Solvent & Solubility
1M HCl : 33.33 mg/mL (148.66 mM; ultrasonic and adjust pH to 1 with HCl)
DMSO : 1 mg/mL (4.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. S Okuda, et al. 3-Hydroxykynurenine, an endogenous oxidative stress generator, causes neuronal cell death with apoptotic features and region selectivity. J Neurochem. 1998 Jan;70(1):299-307. [Content Brief]
[2]. Sarah S Zaher,et al. 3-hydroxykynurenine suppresses CD4+ T-cell proliferation, induces T-regulatory-cell development, and prolongs corneal allograft survival. Invest Ophthalmol Vis Sci. 2011 Apr 22;52(5):2640-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / 1M HCl | 1 mM | 4.4601 mL | 22.3005 mL | 44.6010 mL | 111.5026 mL |
| 1M HCl | 5 mM | 0.8920 mL | 4.4601 mL | 8.9202 mL | 22.3005 mL |
| 10 mM | 0.4460 mL | 2.2301 mL | 4.4601 mL | 11.1503 mL | |
| 15 mM | 0.2973 mL | 1.4867 mL | 2.9734 mL | 7.4335 mL | |
| 20 mM | 0.2230 mL | 1.1150 mL | 2.2301 mL | 5.5751 mL | |
| 25 mM | 0.1784 mL | 0.8920 mL | 1.7840 mL | 4.4601 mL | |
| 30 mM | 0.1487 mL | 0.7434 mL | 1.4867 mL | 3.7168 mL | |
| 40 mM | 0.1115 mL | 0.5575 mL | 1.1150 mL | 2.7876 mL | |
| 50 mM | 0.0892 mL | 0.4460 mL | 0.8920 mL | 2.2301 mL | |
| 60 mM | 0.0743 mL | 0.3717 mL | 0.7434 mL | 1.8584 mL | |
| 80 mM | 0.0558 mL | 0.2788 mL | 0.5575 mL | 1.3938 mL | |
| 100 mM | 0.0446 mL | 0.2230 mL | 0.4460 mL | 1.1150 mL |
3-Hydroxykynurenine Related Classifications
HY-113294 Related Classifications
Powered by Bioz