3-O-Caffeoylshikimic acid
Based on 1 Customer Validation
3-O-Caffeoylshikimic acid is a caffeoylshikimic acid substance and phenolic acid that widely exists in the aerial parts of Equisetum arvense L. and Smilax glabra. During sulfur fumigation processing, 3-O-Caffeoylshikimic acid undergoes chemical transformation to produce 3-O-caffeoylshikimic acid sulfate and 3-O-caffeoylshikimic acid sulfite. At a high concentration (40 μM), 3-O-Caffeoylshikimic acid exhibits cytotoxic activity against human keratinocytes.
For research use only. We do not sell to patients.
- Purity : 70.0%
- CAS No.: 180981-12-8
- Formula: C16H16O8
- Molecular Weight:336.29
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
3-O-Caffeoylshikimic acid (compound 9)(40-80 μM; 24 h) is non-cytotoxic to HaCaT human keratinocytes at 40 μM, exhibits cytotoxicity at 80 μM, and does not significantly improve TNFα/IFNγ-induced cell viability reduction at 40 μM[1].
3-O-Caffeoylshikimic acid (40 μM; 2 h pre-treatment, followed by 10 min TNFα/IFNγ stimulation) does not significantly inhibit activation of NF-κB, AP-1 (c-Jun), or STAT1 pro-inflammatory transcription factors in TNFα/IFNγ-stimulated HaCaT human keratinocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HaCaT human keratinocytes
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Concentration:40 μM, 80 μM (without TNFα/IFNγ stimulation); 40 μM (with TNFα/IFNγ stimulation)
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Incubation Time:24 h
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Result:Showed no cytotoxicity in HaCaT cells without TNFα/IFNγ stimulation at 40 μM.
Exhibited cytotoxicity in HaCaT cells without TNFα/IFNγ stimulation at 80 μM.
Did not significantly improve the TNFα/IFNγ-induced reduction in cell viability at 40 μM.
Chemical Information
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CAS No. 180981-12-8
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Appearance Solid
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Molecular Weight 336.29
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Formula C16H16O8
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Color White to off-white
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SMILES
O=C(O[C@H]1[C@@H](O)[C@@H](CC(C(O)=O)=C1)O)/C=C/C2=CC(O)=C(C=C2)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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