3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate
3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate is a multiflorane triterpene ester found in the seeds of Trichosanthes kirilowii and non-cytotoxic agent.3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate lacks marked cytotoxic activity against cancer cells.
For research use only. We do not sell to patients.
- CAS No.: 873001-55-9
- Formula: C44H56O5
- Molecular Weight:664.91
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate (Compound 3) (3 d+6 h) shows no marked cytotoxicity against the K562 cell line under the tested assay conditions[1].
3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate (Compound 3) can be isolated from the CHCl3 extract of Trichosanthes kirilowii seeds via sequential silica gel column chromatography, yielding 50 mg from 2.5 kg of dried seeds[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 873001-55-9
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Molecular Weight 664.91
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Formula C44H56O5
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SMILES
C[C@@]12[C@]([C@@]3([H])[C@@](CC1)(CC[C@](C)(C3)COC(C4=CC=CC=C4)=O)C)(CCC5=C2C(C[C@]6([H])[C@@]5(CC[C@H](C6(C)C)OC(C7=CC=CC=C7)=O)C)=O)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate Related Classifications
HY-N17942 Related Classifications
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Keywords
- 3a-3,29-Dihydroxy-7-oxomultiflor-8-ene-3,29-diyldibenzoate
- 873001-55-9
- Others
- NMR spectroscopic analysis
- HR-MALDI-MS analysis
- multiflorane triterpene ester
- silica gel column chromatography
- multiflorane triterpene core
- IR spectroscopic analysis
- K562 cell line
- Trichosanthes kirilowii
- cancer cells
- Inhibitor
- inhibitor
- inhibit