4',5'-Dibromofluorescein
Based on 1 Customer Validation
4',5'-Dibromofluorescein (Eosinic acid) is a fluorescein derivative that can used as a ligand for studying of protein properties by spectral analysis.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 596-03-2
- Formula: C20H10Br2O5
- Molecular Weight:490.10
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Chemical Information
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CAS No. 596-03-2
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Appearance Solid
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Molecular Weight 490.10
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Formula C20H10Br2O5
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Color Orange to red
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4Br)C(Br)=C(O)C=C3)C5=C1C=CC=C5
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Synonyms
Eosinic acid; Solvent Red 72
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL (255.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0404 mL | 10.2020 mL | 20.4040 mL | 51.0100 mL |
| 5 mM | 0.4081 mL | 2.0404 mL | 4.0808 mL | 10.2020 mL | |
| 10 mM | 0.2040 mL | 1.0202 mL | 2.0404 mL | 5.1010 mL | |
| 15 mM | 0.1360 mL | 0.6801 mL | 1.3603 mL | 3.4007 mL | |
| 20 mM | 0.1020 mL | 0.5101 mL | 1.0202 mL | 2.5505 mL | |
| 25 mM | 0.0816 mL | 0.4081 mL | 0.8162 mL | 2.0404 mL | |
| 30 mM | 0.0680 mL | 0.3401 mL | 0.6801 mL | 1.7003 mL | |
| 40 mM | 0.0510 mL | 0.2550 mL | 0.5101 mL | 1.2752 mL | |
| 50 mM | 0.0408 mL | 0.2040 mL | 0.4081 mL | 1.0202 mL | |
| 60 mM | 0.0340 mL | 0.1700 mL | 0.3401 mL | 0.8502 mL | |
| 80 mM | 0.0255 mL | 0.1275 mL | 0.2550 mL | 0.6376 mL | |
| 100 mM | 0.0204 mL | 0.1020 mL | 0.2040 mL | 0.5101 mL |