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  3. Fluorescent Dye
  4. 4',5'-Dibromofluorescein

4',5'-Dibromofluorescein  (Synonyms: Eosinic acid; Solvent Red 72)

Cat. No.: HY-114907 Purity: ≥95.0%
COA Handling Instructions

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4',5'-Dibromofluorescein (Eosinic acid) is a fluorescein derivative that can used as a ligand for studying of protein properties by spectral analysis.

For research use only. We do not sell to patients.

4',5'-Dibromofluorescein Chemical Structure

4',5'-Dibromofluorescein Chemical Structure

CAS No. : 596-03-2

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Solid + Solvent
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

4',5'-Dibromofluorescein Related Antibodies

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Description

4',5'-Dibromofluorescein (Eosinic acid) is a fluorescein derivative that can used as a ligand for studying of protein properties by spectral analysis[1].

Molecular Weight

490.10

Formula

C20H10Br2O5
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4Br)C(Br)=C(O)C=C3)C5=C1C=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (127.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0404 mL 10.2020 mL 20.4040 mL
5 mM 0.4081 mL 2.0404 mL 4.0808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥95.0%

COA (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0404 mL 10.2020 mL 20.4040 mL 51.0100 mL
5 mM 0.4081 mL 2.0404 mL 4.0808 mL 10.2020 mL
10 mM 0.2040 mL 1.0202 mL 2.0404 mL 5.1010 mL
15 mM 0.1360 mL 0.6801 mL 1.3603 mL 3.4007 mL
20 mM 0.1020 mL 0.5101 mL 1.0202 mL 2.5505 mL
25 mM 0.0816 mL 0.4081 mL 0.8162 mL 2.0404 mL
30 mM 0.0680 mL 0.3401 mL 0.6801 mL 1.7003 mL
40 mM 0.0510 mL 0.2550 mL 0.5101 mL 1.2752 mL
50 mM 0.0408 mL 0.2040 mL 0.4081 mL 1.0202 mL
60 mM 0.0340 mL 0.1700 mL 0.3401 mL 0.8502 mL
80 mM 0.0255 mL 0.1275 mL 0.2550 mL 0.6376 mL
100 mM 0.0204 mL 0.1020 mL 0.2040 mL 0.5101 mL
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4',5'-Dibromofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4',5'-Dibromofluorescein596-03-2Eosinic acid Solvent Red 72Solvent Red72Solvent Red 72Solvent Red-72Fluorescent DyeInhibitorinhibitorinhibit

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