4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate
Based on 1 publication(s) in Google Scholar
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate is a fluorescent dye. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate undergoes desulphation by galactose-6-sulphate sulphatase to form 4-methylumbelliferyl-β-D-galactopyranoside, which is cleaved by β-galactosidase to release fluorescent 4-methylumbelliferone. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate interacts with N-acetylgalactosamine-6-sulfate sulfatase (GALNS) via hydrogen bonds, electrostatic interactions, and steric interactions. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate serves as a substrate in assays measuring galactose-6-sulphate sulphatase and GALNS activity. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate can be used for the research of Morquio disease type A (mucopolysaccharidosis IV A).
For research use only. We do not sell to patients.
- CAS No.: 126938-14-5
- Formula: C16H18O11S
- Molecular Weight:418.37
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate
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Biological Activity
Description
In Vitro
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate acts as a fluorogenic substrate for Gal-6S sulphatase, enabling sensitive detection of enzyme activity in normal human leucocytes and fibroblasts[1].
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate binds to wild-type human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme with an affinity energy of -121.685 kJ/mol, forming hydrogen, electrostatic, and steric interactions with specific active cavity residues[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
425
Excitation (Ex)
318
Chemical Information
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CAS No. 126938-14-5
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Appearance Solid
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Molecular Weight 418.37
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Formula C16H18O11S
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Color White to off-white
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SMILES
CC(C1=CC=C(O[C@@H]2O[C@@H]([C@@H]([C@@H]([C@H]2O)O)O)COS(=O)(O)=O)C=C1O3)=CC3=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. van Diggelen OP, et al. A fluorimetric enzyme assay for the diagnosis of Morquio disease type A (MPS IV A). Clin Chim Acta. 1990;187(2):131-139. [Content Brief]
[2]. Olarte-Avellaneda S, et al. Computational analysis of human N-acetylgalactosamine-6-sulfate sulfatase enzyme: an update in genotype-phenotype correlation for Morquio A. Mol Biol Rep. 2014 Nov;41(11):7073-88. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate Related Classifications
HY-D1633 Related Classifications
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