4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate 

4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate is a fluorescent dye. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate undergoes desulphation by galactose-6-sulphate sulphatase to form 4-methylumbelliferyl-β-D-galactopyranoside, which is cleaved by β-galactosidase to release fluorescent 4-methylumbelliferone. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate interacts with N-acetylgalactosamine-6-sulfate sulfatase (GALNS) via hydrogen bonds, electrostatic interactions, and steric interactions. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate serves as a substrate in assays measuring galactose-6-sulphate sulphatase and GALNS activity. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate can be used for the research of Morquio disease type A (mucopolysaccharidosis IV A)^[1][2].

4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate acts as a fluorogenic substrate for Gal-6S sulphatase, enabling sensitive detection of enzyme activity in normal human leucocytes and fibroblasts^[1].
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate binds to wild-type human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme with an affinity energy of -121.685 kJ/mol, forming hydrogen, electrostatic, and steric interactions with specific active cavity residues^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

418.37

C₁₆H₁₈O₁₁S

126938-14-5

Solid

White to off-white

425

318

CC(C1=CC=C(O[C@@H]2O[C@@H]([C@@H]([C@@H]([C@H]2O)O)O)COS(=O)(O)=O)C=C1O3)=CC3=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. van Diggelen OP, et al. A fluorimetric enzyme assay for the diagnosis of Morquio disease type A (MPS IV A). Clin Chim Acta. 1990;187(2):131-139.  [Content Brief]

  [2]. Olarte-Avellaneda S, et al. Computational analysis of human N-acetylgalactosamine-6-sulfate sulfatase enzyme: an update in genotype-phenotype correlation for Morquio A. Mol Biol Rep. 2014 Nov;41(11):7073-88.  [Content Brief]