5,6-Dihydrouridine
Based on 2 publication(s) in Google Scholar
5,6-Dihydrouridine is a modified base found in conserved positions in the D-loop of tRNA in Bacteria, Eukaryota, and some Archaea.
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- Pureza: 99.58%
- No. CAS: 5627-05-4
- Fòrmula: C9H14N2O6
- Peso molecular:246.22
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Publications Citing Use of MedChemExpress (MCE) 5,6-Dihydrouridine
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Actividad biológica
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Human Endogenous Metabolite |
5,6-Dihydrouridine (Dihydrouridine) is one of the posttranscriptionally modified nucleosides. It is a product of the reduction of uridine (U), and can be further modified to 5-methyldihydrouridine (m5D). 5,6-Dihydrouridine is commonly present in the tRNA from Bacteria, Eukaryota, and some Archaea. It is identified in six positions in the “D-loop” of the tRNA (16, 17, 20a, 20b) and in position 47 in the variable loop[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 5627-05-4
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Appearance Solid
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Peso molecular 246.22
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Fòrmula C9H14N2O6
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Color White to off-white
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SMILES
O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N(C(N2)=O)CCC2=O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Talanta
High-throughput profiling of RNA modifications by ultra-performance liquid chromatography coupled to complementary mass spectrometry: Methods, quality control, and applications. [Abstract]2023 Oct 1:263:124697. PMID: 37262985 -
Int J Mol Sci
Untargeted Metabolomics Reveals Molecular Effects of Ketogenic Diet on Healthy and Tumor Xenograft Mouse Models. [Abstract]2019 Aug 8;20(16):3873. PMID: 31398922
Solvente y solubilidad
H2O : 100 mg/mL (406.14 mM; Need ultrasonic)
DMSO : 35.71 mg/mL (145.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 65 mg/mL (263.99 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.0614 mL | 20.3070 mL | 40.6141 mL | 101.5352 mL |
| 5 mM | 0.8123 mL | 4.0614 mL | 8.1228 mL | 20.3070 mL | |
| 10 mM | 0.4061 mL | 2.0307 mL | 4.0614 mL | 10.1535 mL | |
| 15 mM | 0.2708 mL | 1.3538 mL | 2.7076 mL | 6.7690 mL | |
| 20 mM | 0.2031 mL | 1.0154 mL | 2.0307 mL | 5.0768 mL | |
| 25 mM | 0.1625 mL | 0.8123 mL | 1.6246 mL | 4.0614 mL | |
| 30 mM | 0.1354 mL | 0.6769 mL | 1.3538 mL | 3.3845 mL | |
| 40 mM | 0.1015 mL | 0.5077 mL | 1.0154 mL | 2.5384 mL | |
| 50 mM | 0.0812 mL | 0.4061 mL | 0.8123 mL | 2.0307 mL | |
| 60 mM | 0.0677 mL | 0.3385 mL | 0.6769 mL | 1.6923 mL | |
| 80 mM | 0.0508 mL | 0.2538 mL | 0.5077 mL | 1.2692 mL | |
| 100 mM | 0.0406 mL | 0.2031 mL | 0.4061 mL | 1.0154 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5,6-Dihydrouridine Related Classifications
HY-113047 Related Classifications
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