5-Nitrobenzimidazole
Based on 1 Customer Validation
5-nitrobenzimidazole is a genotoxic nitro compound and 6-aminopurine (HY-B0152) analogue. 5-nitrobenzimidazole shows a slight inhibitory effect on xanthine oxidase, with an IC50 of 86.84 μM. 5-nitrobenzimidazole can be used in the research of hyperuricemia.
For research use only. We do not sell to patients.
- Purity : 99.74%
- CAS No.: 94-52-0
- Formula: C7H5N3O2
- Molecular Weight:163.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-W008378
5-Methoxy-2-benzimidazolethiolBenzimidazole Derivative -
HY-W009906
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HY-20737
2-BromobenzimidazoleBiochemical Reagent -
HY-W029043
2-(4-Pyridyl)benzimidazoleMetal-organic Framework -
HY-W015575
2-ChlorobenzimidazoleDurg Intermediate -
HY-W008378R
5-Methoxy-2-benzimidazolethiol (Standard)Reference Standard
Biological Activity
Description
IC50 & Target
IC50: 86.84 μM (xanthine oxidase)[1]
In Vitro
5-nitrobenzimidazole shows a slight inhibitory effect on xanthine oxidase, with an IC50 of 86.84 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 94-52-0
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Appearance Solid
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Molecular Weight 163.14
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Formula C7H5N3O2
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Color White to yellow
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SMILES
O=[N+]([O-])C1=CC2=C(N=CN2)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (612.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 6.1297 mL | 30.6485 mL | 61.2970 mL | 153.2426 mL |
| 5 mM | 1.2259 mL | 6.1297 mL | 12.2594 mL | 30.6485 mL | |
| 10 mM | 0.6130 mL | 3.0649 mL | 6.1297 mL | 15.3243 mL | |
| 15 mM | 0.4086 mL | 2.0432 mL | 4.0865 mL | 10.2162 mL | |
| 20 mM | 0.3065 mL | 1.5324 mL | 3.0649 mL | 7.6621 mL | |
| 25 mM | 0.2452 mL | 1.2259 mL | 2.4519 mL | 6.1297 mL | |
| 30 mM | 0.2043 mL | 1.0216 mL | 2.0432 mL | 5.1081 mL | |
| 40 mM | 0.1532 mL | 0.7662 mL | 1.5324 mL | 3.8311 mL | |
| 50 mM | 0.1226 mL | 0.6130 mL | 1.2259 mL | 3.0649 mL | |
| 60 mM | 0.1022 mL | 0.5108 mL | 1.0216 mL | 2.5540 mL | |
| 80 mM | 0.0766 mL | 0.3831 mL | 0.7662 mL | 1.9155 mL | |
| 100 mM | 0.0613 mL | 0.3065 mL | 0.6130 mL | 1.5324 mL |
5-Nitrobenzimidazole Related Classifications
HY-W010132 Related Classifications
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