6-epi-Paederosidic acid
6-epi-Paederosidic acid is a cyclopentanoid monoterpene glycoside found in the aerial parts of Paederia foetida L. 6-epi-Paederosidic acid does not exhibit cytotoxic activity against human tumor cells, hepatoprotective activity against APAP (HY-66005)-induced cellular toxicity, or inhibitory activity against LPS (HY-D1056)-induced nitric oxide production in murine microglia.
For research use only. We do not sell to patients.
- CAS No.: 435278-17-4
- Formula: C18H24O12S
- Molecular Weight:464.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
6-epi-Paederosidic acid (Compound 8) (0.1-10.0 μg/mL; 96 h) shows no cytotoxic activity against HCT116, MCF7, BGC823, HepG2, PC9, and PANC1 human tumor cell lines, with an IC50 >10 μM[1].
6-epi-Paederosidic acid (10 μM; 24 h) exhibits no significant hepatoprotective activity against APAP (HY-66005)-induced toxicity in HepG2 cells[1].
6-epi-Paederosidic acid (10 μM; 24 h) does not inhibit LPS (HY-D1056)-induced nitric oxide production in BV2 microglial cells, with an IC50 >10 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HCT116, MCF7, BGC823, HepG2, PC9, PANC1
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Concentration:0.1, 1.0, and 10.0 μg/mL
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Incubation Time:96 h
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Result:Showed no cytotoxic activity, with an IC50 value greater than 10 μM.
Chemical Information
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CAS No. 435278-17-4
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Molecular Weight 464.44
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Formula C18H24O12S
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SMILES
OC(C1=CO[C@H]([C@@]2([H])[C@]1([H])[C@@H](C=C2COC(SC)=O)O)O[C@@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
6-epi-Paederosidic acid Related Classifications
HY-N6998A Related Classifications
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