7-Methyl-diguanosine triphosphate ammonium  (Synonyms: m7Gp3G ammonium)

7-Methyl-diguanosine triphosphate (m7Gp3G) ammonium is an mRNA cap structure analog that inhibits in vitro protein synthesis by binding to the translation initiation complex. 7-Methyl-diguanosine triphosphate ammonium binds to eIF4E, promotes cap-dependent translation initiation, stabilizes mRNA, and acts as a translation enhancer. 7-Methyl-diguanosine triphosphate ammonium can be used to prepare synthetic capped RNA transcripts for studies related to mRNA translation, splicing, turnover, and intracellular transport^[1][2].

7-Methyl-diguanosine triphosphate ammonium inhibits cap-dependent translation in a rabbit reticulocyte lysate system, with an inhibition constant (K_i) of 17 μM^[1].
7-Methyl-diguanosine triphosphate (5 μg per 107 cells; electroporation; single dose) ammonium confers differential in vivo stability to luciferase mRNAs based on their 3'-terminal structures, with 7-Methyl-diguanosine triphosphate ammonium-capped Luc-G16 mRNA having the longest half-life (246 min), and 7-Methyl-diguanosine triphosphate ammonium-capped Luc-A60 mRNA undergoing predominant 5'→3' degradation^[1].
7-Methyl-diguanosine triphosphate ammonium exhibits high affinity for the eukaryotic initiation factor eIF4E and can be used to generate affinity media for capturing and purifying initiation factors^[2].
The affinity of 7-Methyl-diguanosine triphosphate ammonium for the poly-specific ribonuclease (PARN) polypeptide is measured via fluorescence titration ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

836.49

C₂₁H₃₅N₁₂O₁₈P₃

75252-12-9

O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C2NC(N)=NC3=O)O1)OP(OP(OC[C@H]4O[C@@H](N5C(NC(N)=N6)=C([N+](C)=C5)C6=O)[C@H](O)[C@@H]4O)([O-])=O)(O)=O.N.N

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• [1]. Grudzien E, et al. Differential inhibition of mRNA degradation pathways by novel cap analogs. J Biol Chem. 2006;281(4):1857-1867.  [Content Brief]

  [2]. Grudzien-Nogalska E, et al. Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability. Methods Enzymol. 2007;431:203-227.  [Content Brief]