7-Methyl-diguanosine triphosphate ammonium solution (100 mM)
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7-Methyl-diguanosine triphosphate (m7Gp3G) ammonium is an mRNA cap structure analog that inhibits in vitro protein synthesis by binding to the translation initiation complex. 7-Methyl-diguanosine triphosphate ammonium binds to eIF4E, promotes cap-dependent translation initiation, stabilizes mRNA, and acts as a translation enhancer. 7-Methyl-diguanosine triphosphate ammonium can be used to prepare synthetic capped RNA transcripts for studies related to mRNA translation, splicing, turnover, and intracellular transport.
For research use only. We do not sell to patients.
- Purity : 98.83%
- CAS No.: 75252-12-9
- Formula: C21H35N12O18P3
- Molecular Weight:836.49
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
7-Methyl-diguanosine triphosphate ammonium inhibits cap-dependent translation in a rabbit reticulocyte lysate system, with an inhibition constant (Ki) of 17 μM[1].
7-Methyl-diguanosine triphosphate (5 μg per 107 cells; electroporation; single dose) ammonium confers differential in vivo stability to luciferase mRNAs based on their 3'-terminal structures, with 7-Methyl-diguanosine triphosphate ammonium-capped Luc-G16 mRNA having the longest half-life (246 min), and 7-Methyl-diguanosine triphosphate ammonium-capped Luc-A60 mRNA undergoing predominant 5'→3' degradation[1].
7-Methyl-diguanosine triphosphate ammonium exhibits high affinity for the eukaryotic initiation factor eIF4E and can be used to generate affinity media for capturing and purifying initiation factors[2].
The affinity of 7-Methyl-diguanosine triphosphate ammonium for the poly-specific ribonuclease (PARN) polypeptide is measured via fluorescence titration [2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 75252-12-9
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Appearance Liquid
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Molecular Weight 836.49
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Formula C21H35N12O18P3
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Color White to off-white
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SMILES
O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C2NC(N)=NC3=O)O1)OP(OP(OC[C@H]4O[C@@H](N5C(NC(N)=N6)=C([N+](C)=C5)C6=O)[C@H](O)[C@@H]4O)([O-])=O)(O)=O.N.N
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Synonyms
m7Gp3G ammonium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Grudzien E, et al. Differential inhibition of mRNA degradation pathways by novel cap analogs. J Biol Chem. 2006;281(4):1857-1867. [Content Brief]
[2]. Grudzien-Nogalska E, et al. Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability. Methods Enzymol. 2007;431:203-227. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
7-Methyl-diguanosine triphosphate ammonium solution (100 mM) Related Classifications
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