7-Methyl-diguanosine triphosphate
7-Methyl-diguanosine triphosphate (m7Gp3G) is an mRNA cap structure analog that inhibits in vitro protein synthesis by binding to the translation initiation complex. 7-Methyl-diguanosine triphosphate binds to eIF4E, promotes cap-dependent translation initiation, stabilizes mRNA, and acts as a translation enhancer. 7-Methyl-diguanosine triphosphate can be used to prepare synthetic capped RNA transcripts for studies related to mRNA translation, splicing, turnover, and intracellular transport.
For research use only. We do not sell to patients.
- CAS No.: 62828-64-2
- Formula: C21H29N10O18P3
- Molecular Weight:802.43
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
7-Methyl-diguanosine triphosphate inhibits cap-dependent translation in a rabbit reticulocyte lysate system, with an inhibition constant (Ki) of 17 μM[1].
7-Methyl-diguanosine triphosphate (5 μg per 107 cells; electroporation; single dose) confers differential in vivo stability to luciferase mRNAs based on their 3'-terminal structures, with 7-Methyl-diguanosine triphosphate-capped Luc-G16 mRNA having the longest half-life (246 min), and 7-Methyl-diguanosine triphosphate-capped Luc-A60 mRNA undergoing predominant 5'→3' degradation[1].
7-Methyl-diguanosine triphosphate exhibits high affinity for the eukaryotic initiation factor eIF4E and can be used to generate affinity media for capturing and purifying initiation factors[2].
The affinity of 7-Methyl-diguanosine triphosphate for the poly-specific ribonuclease (PARN) polypeptide is measured via fluorescence titration[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 62828-64-2
-
Molecular Weight 802.43
-
Formula C21H29N10O18P3
-
SMILES
O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C2NC(N)=NC3=O)O1)OP(OP(OC[C@H]4O[C@@H](N5C(NC(N)=N6)=C([N+](C)=C5)C6=O)[C@H](O)[C@@H]4O)([O-])=O)(O)=O
-
Synonyms
m7Gp3G
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Grudzien E, et al. Differential inhibition of mRNA degradation pathways by novel cap analogs. J Biol Chem. 2006;281(4):1857-1867. [Content Brief]
[2]. Grudzien-Nogalska E, et al. Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability. Methods Enzymol. 2007;431:203-227. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
7-Methyl-diguanosine triphosphate Related Classifications
HY-139098 Related Classifications
Powered by Bioz