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Atrimustine  (Synonyms: Bestrabucil; KM2210)

Cat. No.: HY-101604 Purity: 98.88%
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Atrimustine is a conjugate of chlorambucil and β-estradiol benzoate with the antitumor activity.

For research use only. We do not sell to patients.

Atrimustine Chemical Structure

Atrimustine Chemical Structure

CAS No. : 75219-46-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 476 In-stock
Solution
10 mM * 1 mL in DMSO USD 476 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 950 In-stock
50 mg USD 1450 In-stock
100 mg USD 2250 In-stock
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Atrimustine Related Antibodies

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Description

Atrimustine is a conjugate of chlorambucil and β-estradiol benzoate with the antitumor activity.

In Vitro

Atrimustine (Bestrabucil), a conjugate of chlorambucil and β-estradiol benzoate, has high affinity for tumor cells and enhances the antitumor activity of chlorambucil[1].The effect of Atrimustine (Bestrabucil), a benzoate of an estradiol-chlorambucil conjugate, is examined on the production of growth factor(s) by Shionogi carcinoma 115 (SC-115) cells, an androgen-responsive cultured cancer cell line. At Atrimustine concentrations of 100 nM-10 μM, concentration-dependent inhibition of growth factor production by SC-115 cells can be demonstrated by 3H-thymidine uptake assay[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Atrimustine Related Antibodies
Molecular Weight

720.72

Formula

C41H47Cl2NO6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1([C@H]2OC(COC(CCCC3=CC=C(N(CCCl)CCCl)C=C3)=O)=O)[C@](CC2)([H])[C@@](CCC4=CC(OC(C5=CC=CC=C5)=O)=CC=C64)([H])[C@]6([H])CC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (23.13 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3875 mL 6.9375 mL 13.8750 mL
5 mM 0.2775 mL 1.3875 mL 2.7750 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[2]

In the culture system containing 10 nM Testosterone, Atrimustine (Bestrabucil) exhibits a statistically significant inhibition of SC-115 cell growth at concentrations of 10 μM and 1 μM. Mixtures of Estradiol and Chlorambucil also significantly inhibit the growth of SC- 115 cells at concentrations of 1 μM and 100 nM. The inhibitory effect at day 7 of culture is regarded as similar for 10 μM Atrimustine and 1 μM of the mixture. At 10 μM, Atrimustine inhibits the growth of SC-115 cells to the sarve degree as the control culture without Testosterone and this concentration of Atrimustine is taken as the IC50. Thus, in experiment 2, 10 μM Atrimustine is used in the CM of SC-115 cells to study the inhibition of growth by Atrimustine [2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3875 mL 6.9375 mL 13.8750 mL 34.6875 mL
5 mM 0.2775 mL 1.3875 mL 2.7750 mL 6.9375 mL
10 mM 0.1388 mL 0.6938 mL 1.3875 mL 3.4688 mL
15 mM 0.0925 mL 0.4625 mL 0.9250 mL 2.3125 mL
20 mM 0.0694 mL 0.3469 mL 0.6938 mL 1.7344 mL
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Atrimustine Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atrimustine75219-46-4Bestrabucil KM2210KM2210KM 2210KM-2210OthersInhibitorinhibitorinhibit

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