Ergosterol
Based on 3 publication(s) in Google Scholar
Ergosterol is the primary sterol found in fungi, with antioxidative, anti-proliferative, and anti-inflammatory effects.
For research use only. We do not sell to patients.
- Purity : 99.91%
- CAS No.: 57-87-4
- Formula: C28H44O
- Molecular Weight:396.65
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-N0131
StigmasterolPlant Sterol -
HY-N0322
Cholesterol (from animal)ERRα Agonist -
HY-113279
7-DehydrocholesterolEndogenous Metabolite -
HY-N0171A
Beta-Sitosterol (purity>98%)Dietary Plant Sterols -
HY-W020033
Lanosterol -
HY-76542
Vitamin D2Vitamin D Supplement -
HY-N0181R
Ergosterol (Standard)Endogenous Metabolite -
HY-14393
EmodinNatural Anthraquinone -
HY-N0148
RutinCBR1 Inhibitor -
HY-N4103
FucosterolBioactive Sterol -
HY-100218A
RSL3GPX4 Inhibitor -
HY-N0140
Ursolic acidAutophagy Inducer
Publications Citing Use of MedChemExpress (MCE) Ergosterol
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Customer Validation & Images
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
19.2 μM
Compound: 19
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Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
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[PMID: 33691166] |
| BV-2 | IC50 |
30.6 μM
Compound: 20
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Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method
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[PMID: 21028898] |
| COLO 205 | IC50 |
4.9 μM
Compound: 6
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Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
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[PMID: 35293752] |
| COLO 320 | IC50 |
6.5 μM
Compound: 6
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Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
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[PMID: 35293752] |
| HeLa | IC50 |
0.91 μg/mL
Compound: 1
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Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay
Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay
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10.1007/s00044-010-9409-z |
| L5178Y | EC50 |
12.6 μM
Compound: 7
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Cytotoxicity against mouse L5178Y cells by MTT assay
Cytotoxicity against mouse L5178Y cells by MTT assay
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[PMID: 27070198] |
| MRC5 | IC50 |
0.5 μM
Compound: 6
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Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
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[PMID: 35293752] |
| NCI-H157 | IC50 |
23.3 μM
Compound: 19
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Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
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[PMID: 33691166] |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 57-87-4
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Appearance Solid
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Molecular Weight 396.65
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Formula C28H44O
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Color White to yellow
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SMILES
C[C@H](C(C)C)/C=C/[C@@H](C)[C@H]1CC[C@@]2([H])C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Structure Classification
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Initial Source
fungi
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Nature
2024 Feb;626(7998):411-418. PMID: 38297130
Ergosterol purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418. [Abstract]
Representative autoxidation of STY-BODIPY (1 μM) and EggPC liposome containing 5% 7-DHC, 5% Ergosterol, 5% Stigmasterol and 5% Cholesterol respectively.
Ergosterol purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418. [Abstract]
Cell viability of HEK293T cells treated with RSL3 for 6-8 h after pretreatment of 7-DHC (25 μM), Ergosterol (25 μM), Stigmasterol (25 μM) or Cholesterol (25 μM) for 24 h.
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Commun Biol
The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi. [Abstract]2023 Jan 3;6(1):1. PMID: 36596887
Ergosterol purchased from MedChemExpress. Usage Cited in: Commun Biol. 2023 Jan 3;6(1):1. [Abstract]
The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi.
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Virol Sin
Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]2024 Oct;39(5):802-811. PMID: 39168248
Solvent & Solubility
In Vitro:
Ethanol : 2 mg/mL (5.04 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.5211 mL | 12.6056 mL | 25.2111 mL | 63.0279 mL |
| 5 mM | 0.5042 mL | 2.5211 mL | 5.0422 mL | 12.6056 mL |
Ergosterol Related Classifications
HY-N0181 Related Classifications
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