2. Anti-infection
  3. Virus Protease
  4. NGI-1

NGI-1  (Synonyms: ML414)

Cat. No.: HY-117383 Purity: 99.90%
COA Handling Instructions

NGI-1 (ML414) is a potent oligosaccharyltransferase (OST) inhibitor, directly targeting and blocking the function of the OST catalytic subunits STT3A and STT3B. NGI-1 is a cell permeable inhibitor and can effectively reduce virus infectivity without affecting cell viability.

For research use only. We do not sell to patients.

NGI-1 Chemical Structure

NGI-1 Chemical Structure

CAS No. : 790702-57-7

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Solution
10 mM * 1 mL in DMSO USD 77 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
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Solid
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10 mg USD 110 In-stock
50 mg USD 290 In-stock
100 mg USD 490 In-stock
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Based on 4 publication(s) in Google Scholar

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Description

NGI-1 (ML414) is a potent oligosaccharyltransferase (OST) inhibitor, directly targeting and blocking the function of the OST catalytic subunits STT3A and STT3B[1]. NGI-1 is a cell permeable inhibitor and can effectively reduce virus infectivity without affecting cell viability[2].

IC50 & Target

OST[1]
In Vitro

NGI-1 inhibits the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaires its proteolytic cleavage in a dose-dependent manner[1].
NGI-1 blocks EGFR N-linked glycosylation in lung adenocarcinoma cells as assessed. In controls EGFR is biotinylated, consistent with its plasma membrane expression, but in NGI-1 treated cells the EGFR is predominantly found in the non-biotinylated intracellular fraction suggesting a change in cellular localization[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: STT3A-, STT3B- and MAGT1-TUSC3- cells
Concentration: 1, 2, 5 μM
Incubation Time: 36 hours
Result: Inhibited the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaired its proteolytic cleavage in a dose-dependent manner.
Molecular Weight

394.51

Appearance

Solid

Formula

C17H22N4O3S2
CAS No.
SMILES

O=C(NC1=NC=C(C)S1)C2=CC(S(=O)(N(C)C)=O)=CC=C2N3CCCC3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (253.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5348 mL 12.6740 mL 25.3479 mL
5 mM 0.5070 mL 2.5348 mL 5.0696 mL
10 mM 0.2535 mL 1.2674 mL 2.5348 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.90%

COA (250 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

NGI-1790702-57-7ML414NGI1NGI 1ML 414ML-414Virus ProteaseInhibitorinhibitorinhibit

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