2. Anti-infection
  3. Virus Protease
  4. NGI-1

NGI-1 (ML414) is a potent oligosaccharyltransferase (OST) inhibitor, directly targeting and blocking the function of the OST catalytic subunits STT3A and STT3B. NGI-1 is a cell permeable inhibitor and can effectively reduce virus infectivity without affecting cell viability.

For research use only. We do not sell to patients.

CAS No. : 790702-57-7

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Customer Review

Based on 19 publication(s) in Google Scholar

NGI-1 Related Antibodies

Top Publications Citing Use of Products

    NGI-1 purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2025 Oct 22:S2451-9456(25)00306-X.  [Abstract]

    Flow cytometry describes the Annexin V staining of MM cell lines upon NGI-1 (10 μM) treatment resulted in a notable increase in cell death.

    NGI-1 purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2020 Dec;10(12):1872-1893.  [Abstract]

    MDA-MB-468 and SKBR3 cells were treated with 10 μM OST inhibitor NGI-1 for 24 h. The expression of B7-H4 was examined by immunoblotting.

    NGI-1 purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2020 Dec;10(12):1872-1893.  [Abstract]

    Blockade of B7-H4 glycosylation by NGI-1 enhances B7-H4 ubiquitination. 293T cells were transfected with Flag-hB7-H4 in the presence or absence of 10 μM NGI-1 for 24 h. Then Flag-hB7-H4 was immunoprecipitated followed by immunoblotting using antibody against ubiquitin.

    NGI-1 purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2020 Dec;10(12):1872-1893.  [Abstract]

    NGI-1 ( 10 μM, 24 h) significantly increased the DOX-induced expression of cell surface CALR and that of other DAMPs including HSP90 and HSP70 on the cell surface, as detectable by immunofluorescence and flow cytometry.

    NGI-1 purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2020 Dec;10(12):1872-1893.  [Abstract]

    In vivo vaccination experiments showed that combined DOX/NGI-1 (10 μM) treatment yields a more potent vaccine than DOX treatment alone.

    • Biological Activity

    • Purity & Documentation

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    • Customer Review

    Description

    NGI-1 (ML414) is a potent oligosaccharyltransferase (OST) inhibitor, directly targeting and blocking the function of the OST catalytic subunits STT3A and STT3B[1]. NGI-1 is a cell permeable inhibitor and can effectively reduce virus infectivity without affecting cell viability[2].

    IC50 & Target

    OST[1]
    Cellular Effect
    Cell Line Type Value Description References
    Hepatocyte IC50
    20 μM
    Compound: 1
    Cytotoxicity against human Hepatocyte cultured as 3D spheroids incubated for 14 days by CellTiter-Glo luminescence assay
    Cytotoxicity against human Hepatocyte cultured as 3D spheroids incubated for 14 days by CellTiter-Glo luminescence assay
    		[PMID: 39136957]
    In Vitro

    NGI-1 inhibits the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaires its proteolytic cleavage in a dose-dependent manner[1].
    NGI-1 blocks EGFR N-linked glycosylation in lung adenocarcinoma cells as assessed. In controls EGFR is biotinylated, consistent with its plasma membrane expression, but in NGI-1 treated cells the EGFR is predominantly found in the non-biotinylated intracellular fraction suggesting a change in cellular localization[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    NGI-1 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: STT3A-, STT3B- and MAGT1-TUSC3- cells
    Concentration: 1, 2, 5 μM
    Incubation Time: 36 hours
    Result: Inhibited the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaired its proteolytic cleavage in a dose-dependent manner.
    Molecular Weight

    394.51

    Formula

    C17H22N4O3S2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NC1=NC=C(C)S1)C2=CC(S(=O)(N(C)C)=O)=CC=C2N3CCCC3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (63.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5348 mL 12.6740 mL 25.3479 mL
    5 mM 0.5070 mL 2.5348 mL 5.0696 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.97%

    SDS (396 KB)

    COA (251 KB) HNMR (273 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5348 mL 12.6739 mL 25.3479 mL 63.3697 mL
    5 mM 0.5070 mL 2.5348 mL 5.0696 mL 12.6739 mL
    10 mM 0.2535 mL 1.2674 mL 2.5348 mL 6.3370 mL
    15 mM 0.1690 mL 0.8449 mL 1.6899 mL 4.2246 mL
    20 mM 0.1267 mL 0.6337 mL 1.2674 mL 3.1685 mL
    25 mM 0.1014 mL 0.5070 mL 1.0139 mL 2.5348 mL
    30 mM 0.0845 mL 0.4225 mL 0.8449 mL 2.1123 mL
    40 mM 0.0634 mL 0.3168 mL 0.6337 mL 1.5842 mL
    50 mM 0.0507 mL 0.2535 mL 0.5070 mL 1.2674 mL
    60 mM 0.0422 mL 0.2112 mL 0.4225 mL 1.0562 mL
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    NGI-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    NGI-1790702-57-7ML414NGI1NGI 1ML 414ML-414Virus ProteaseInhibitorinhibitorinhibit

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