Cat. No.: HY-P0234

Neurotensin

Name: Neurotensin
Brand: MedChemExpress
SKU: HY-P0234
Price: 66.00 USD
Availability: InStock
Rating: 5 (5 reviews)

5 Cited Publications

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Based on 5 publication(s) in Google Scholar

Neurotensin, a gut tridecapeptide, acts as a potent cellular mitogen for various colorectal and pancreatic cancers which possess high-affinity neurotensin receptors (NTR).

For research use only. We do not sell to patients.

Purity : 99.48%
CAS No.: 39379-15-2
Formula: C₇₈H₁₂₁N₂₁O₂₀
Molecular Weight:1672.92

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Neurotensin Chemical Structure

CAS No.: 39379-15-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Neurotensin, a gut tridecapeptide, acts as a potent cellular mitogen for various colorectal and pancreatic cancers which possess high-affinity neurotensin receptors (NTR).

IC₅₀ & Target

Neurotensin receptors (NTR)^[1]

All Neurotensin Receptor Isoforms :

NTR1
NTR2

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	4.9 x 10^-10 M Compound: Neurotensin	Displacement of [125I]Tyr3-neurotensin from human recombinant NT1 receptor expressed in CHO cells Displacement of [125I]Tyr3-neurotensin from human recombinant NT1 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	4.9 x 10^-10 M Compound: Neurotensin	Displacement of [125I]Tyr3-neurotensin from human recombinant NTS1 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [125I]Tyr3-neurotensin from human recombinant NTS1 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO-K1	EC₅₀	0.31 μM Compound: neurotensin, NT	Agonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as increase in intracellular calcium level by FLIPR assay Agonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as increase in intracellular calcium level by FLIPR assay	[PMID: 18849166]
CHO-K1	IC₅₀	6.03 nM Compound: neurotensin, NT	Antagonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as inhibition of intracellular calcium elevation pretreated 30 mins before with SR-48692 by FLIPR assay Antagonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as inhibition of intracellular calcium elevation pretreated 30 mins before with SR-48692 by FLIPR assay	[PMID: 18849166]
CHO-K1	EC₅₀	0.23 nM Compound: Neurotensin, NT	Agonist activity at NTR1 expressed in CHOK1 cells by calcium mobilization assay Agonist activity at NTR1 expressed in CHOK1 cells by calcium mobilization assay	[PMID: 19195889]
CHO-K1	EC₅₀	0.04 nM Compound: NT	Agonist activity at rat NTS1 stably expressed in CHOK1 cells assessed as induction of calcium release by FLIPR assay Agonist activity at rat NTS1 stably expressed in CHOK1 cells assessed as induction of calcium release by FLIPR assay	[PMID: 24856674]
CHO-K1	IC₅₀	18.5 nM Compound: NT	Antagonist activity at rat NTS2 stably expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay Antagonist activity at rat NTS2 stably expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay	[PMID: 24856674]
CHO-K1	IC₅₀	114 nM Compound: NT	Antagonist activity at rat NTS2 receptor expressed in CHO-K1 cells assessed as inhibition of SR142948a-induced calcium mobilization by FLIPR assay Antagonist activity at rat NTS2 receptor expressed in CHO-K1 cells assessed as inhibition of SR142948a-induced calcium mobilization by FLIPR assay	[PMID: 25157640]
CHO-K1	EC₅₀	>100 nM Compound: NT	Agonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as increase in calcium release by FLIPR assay Agonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as increase in calcium release by FLIPR assay	[PMID: 25499438]
CHO-K1	IC₅₀	42 nM Compound: NT	Antagonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay Antagonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay	[PMID: 25499438]
HEK293	EC₅₀	1.6 nM Compound: Neurotensin	Agonist activity at human NTS1 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity Agonist activity at human NTS1 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity	[PMID: 21446649]
HEK293	EC₅₀	110 nM Compound: Neurotensin	Agonist activity at human NTS2 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity Agonist activity at human NTS2 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity	[PMID: 21446649]
HT-29	IC₅₀	0.2 nM Compound: 1	Binding affinity towards neurotensin receptor in membranes prepared from HT-29 cell line, relative to [111In]-labeled neurotensin peptide Binding affinity towards neurotensin receptor in membranes prepared from HT-29 cell line, relative to [111In]-labeled neurotensin peptide	[PMID: 12852770]
HT-29	IC₅₀	0.62 nM Compound: NT	Displacement of [125I]Tyr3-NT from human NTS1R expressed in HT29 cells Displacement of [125I]Tyr3-NT from human NTS1R expressed in HT29 cells	[PMID: 16854083]
HT-29	EC₅₀	1.98 nM Compound: NT	Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay	[PMID: 19290594]
N1E-115	EC₅₀	2 nM Compound: NT	Agonist activity at NTR1 in mouse N1E-115 cells assessed as increase in intracellular calcium concentration Agonist activity at NTR1 in mouse N1E-115 cells assessed as increase in intracellular calcium concentration	[PMID: 19290594]
WiDr	IC₅₀	0.46 nM Compound: NT	Displacement of [125I]Tyr3-NT from human NTS1R expressed in WiDr cells Displacement of [125I]Tyr3-NT from human NTS1R expressed in WiDr cells	[PMID: 16854083]

In Vitro

Neurotensin induces the expression of MIP-2, MCP-1, IL-1β and TNFα in murine microglial cells and stimulates IL-8 secretion in a non-transformed colon epithelial cell line stably transfected with the NTR. The high-affinity NTR, a member of the G-protein coupled receptor (GPCR) family, is present in a majority of human pancreatic and colorectal cancers, suggesting that Neurotensin (NT) may act in an endocrine fashion to affect tumor growth. Acting through the NTR1, Neurotensin is known to stimulate various signal transduction pathways, including intracellular calcium ([Ca²⁺]_i), the mitogen-activated protein kinases (MAPKs), ERK and JNK, and various PKC isoforms. Treatment of HCT116 cells with Neurotensin (100 nM) significantly increases HCT116 cell migration (~3-fold) compared with vehicle treatment; pretreatment with Curcumin (10 μM) blocksthe stimulatory effect of NT on HCT116 cell migration. ctivation of MEK/ERK by NT and downstream induction of AP-1 transcription factors contributes to the proliferative effects of Neurotensin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Chemical Information

CAS No. 39379-15-2
Appearance Solid
Molecular Weight 1672.92
Formula C₇₈H₁₂₁N₂₁O₂₀
Color White to off-white
Sequence

{Pyr}-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
Sequence Shortening

{Pyr}-LYENKPRRPYIL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (5)

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Talanta

A robust polymetallic-coated sheathless interface with high acid and alkali resistance for coupling capillary electrophoresis with mass spectrometry. [Abstract]

2025 Jan 1:282:127045. PMID: 39418980
ACS Pharmacol Transl Sci

Critical Residues in the Gβ Subunit Mediate Selective Recruitment of the Gαq Heterotrimer to Activated G Protein-Coupled Receptors. [Abstract]

2025 Jun 6;8(7):2048-2060. PMID: 40672669
Exp Dermatol

Neurotensin counteracts hair growth inhibition induced by chronic restraint stress. [Abstract]

2024 Jan;33(1):e14990. PMID: 38071436
J Am Soc Mass Spectrom

Wire Desorption Combined with Electrospray Ionization Mass Spectrometry: Direct Analysis of Small Organic and Large Biological Compounds. [Abstract]

2020 Jul 5. PMID: 32559077
Res Sq

2026 Jun 2.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (29.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (19.92 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.5978 mL

2.9888 mL

5.9776 mL

5 mM

0.1196 mL

0.5978 mL

1.1955 mL

10 mM

0.0598 mL

0.2989 mL

0.5978 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 20 mg/mL (11.96 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang X, et al. Curcumin inhibits neurotensin-mediated interleukin-8 production and migration of HCT116 human colon cancer cells. Clin Cancer Res. 2006 Sep 15;12(18):5346-55. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	0.5978 mL	2.9888 mL	5.9776 mL	14.9439 mL
	5 mM	0.1196 mL	0.5978 mL	1.1955 mL	2.9888 mL
	10 mM	0.0598 mL	0.2989 mL	0.5978 mL	1.4944 mL
	15 mM	0.0399 mL	0.1993 mL	0.3985 mL	0.9963 mL
DMSO	20 mM	0.0299 mL	0.1494 mL	0.2989 mL	0.7472 mL
DMSO	25 mM	0.0239 mL	0.1196 mL	0.2391 mL	0.5978 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Neurotensin

Publications Citing Use of MedChemExpress (MCE) Neurotensin

All Neurotensin Receptor Isoforms