Para-aminoblebbistatin
Based on 1 Customer Validation
Para-aminoblebbistatin is the inhibitor for myosin II. Para-aminoblebbistatin inhibits the ATPase activity of myosin II, thereby blocking the formation and contraction of the actin-myosin network. Para-aminoblebbistatin is a non-fluorescent and photostable Blebbistatin (HY-13813) derivative.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.9%
- CAS. Nr.: 2097734-03-5
- Formel: C18H17N3O2
- Molecular Weight:307.35
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Speicherung:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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HY-109037
MavacamtenCardiac Myosin Modulator -
HY-13441
(-)-BlebbistatinMyosin II ATPase Inhibitor -
HY-120870
para-NitroblebbistatinMyosin II Inhibitor -
HY-107657
(+)-BlebbistatinMyosin II Inhibitor -
HY-13813
BlebbistatinMyosin II Inhibitor -
HY-109037R
Mavacamten (Standard)Cardiac Myosin Modulator -
HY-109128
DanicamtivCardiac Myosin Activator -
HY-129452
(S)-3'-Hydroxy blebbistatinMyosin II Inhibitor -
HY-123634
(S)-3'-AminoblebbistatinMyosin II Inhibitor -
HY-N0282
DemecolcineTubulin Inhibitor -
HY-14233
Omecamtiv mecarbilCardiac Myosin Activator -
HY-15417
ML-7 hydrochlorideMLCK Inhibitor
Biologische Aktivität
Beschreibung
IC50 & Target
Myosin II[1]
In Vitro
Para-aminoblebbistatin inhibits multiple myosin II isoforms, including skeletal muscle, cardiac muscle, non-muscle myosin II. Para-aminoblebbistatin inhibits rabbit skeletal muscle myosin S1 (SkS1) and dictyostelium discoideum myosin II motor domain (DdMD) with IC50 of 1.3 μM and 6.6 μM[1].
Para-aminoblebbistatin (0-50 μM, 72 h) inhibits the proliferation of HeLa cell with an IC50 of 17.8 μM, promotes the cell migration and inhibits the blebbing process of M2 cell[1].
Para-aminoblebbistatin (2-20 μM, 10 stimulations every hour for 4 hours) inhibits the rapid escape response (C-start reflex) of zebrafish embryo, reduces the amplitude of zebrafish embryo myocardial contraction[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats models[3]
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Dosage:16 mg/kg
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Administration:ip, single dose
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Result:Killed rats in 15 minutes.
Chemical Information
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CAS. Nr. 2097734-03-5
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Appearance Solid
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Molecular Weight 307.35
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Formel C18H17N3O2
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Color Orange to red
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SMILES
O=C1[C@@]2(O)C(N(C3=CC=C(N)C=C3)CC2)=NC4=CC=C(C)C=C41
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Lösungsmittel & Löslichkeit
In Vitro:
DMF : 20 mg/mL (65.07 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Várkuti BH, et al. A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci Rep. 2016 May 31;6:26141. [Content Brief]
[2]. Képiró M, et al. para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor. Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. [Content Brief]
[3]. Gyimesi M, et al., Single Residue Variation in Skeletal Muscle Myosin Enables Direct and Selective Drug Targeting for Spasticity and Muscle Stiffness. Cell. 2020 Oct 15;183(2):335-346.e13. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMF | 1 mM | 3.2536 mL | 16.2681 mL | 32.5362 mL | 81.3405 mL |
| 5 mM | 0.6507 mL | 3.2536 mL | 6.5072 mL | 16.2681 mL | |
| 10 mM | 0.3254 mL | 1.6268 mL | 3.2536 mL | 8.1340 mL | |
| 15 mM | 0.2169 mL | 1.0845 mL | 2.1691 mL | 5.4227 mL | |
| 20 mM | 0.1627 mL | 0.8134 mL | 1.6268 mL | 4.0670 mL | |
| 25 mM | 0.1301 mL | 0.6507 mL | 1.3014 mL | 3.2536 mL | |
| 30 mM | 0.1085 mL | 0.5423 mL | 1.0845 mL | 2.7113 mL | |
| 40 mM | 0.0813 mL | 0.4067 mL | 0.8134 mL | 2.0335 mL | |
| 50 mM | 0.0651 mL | 0.3254 mL | 0.6507 mL | 1.6268 mL | |
| 60 mM | 0.0542 mL | 0.2711 mL | 0.5423 mL | 1.3557 mL |
Para-aminoblebbistatin Related Classifications
HY-111474 Related Classifications
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