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Cat. No.: HY-15158A

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SBE13

SBE13 is a potent and selective Plk1 inhibitor, with an IC₅₀ of 200 pM; SBE13 poorly inhibits Plk2 (IC₅₀>66 μM) or Plk3 (IC₅₀=875 nM).

For research use only. We do not sell to patients.

CAS No.: 775294-82-1
Formula: C₂₄H₂₇ClN₂O₄
Molecular Weight:442.94

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

SBE13 Chemical Structure

CAS No.: 775294-82-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

SBE13 is a potent and selective Plk1 inhibitor, with an IC₅₀ of 200 pM; SBE13 poorly inhibits Plk2 (IC₅₀>66 μM) or Plk3 (IC₅₀=875 nM).

IC₅₀ & Target

^[1]

PLK1

200 pM (IC₅₀)

PLK3

875 nM (IC₅₀)

All Polo-like Kinase (PLK) Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	EC₅₀	33 μM Compound: 38; SBE13	Antiproliferative activity against human A-431 cells assessed as reduction in cell proliferation incubated for 72 hrs Antiproliferative activity against human A-431 cells assessed as reduction in cell proliferation incubated for 72 hrs	[PMID: 35878418]
A549	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
Detroit 562	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human Detroit 562 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human Detroit 562 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
HCT-15	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human HCT-15 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human HCT-15 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
HeLa	EC₅₀	18 μM Compound: 13, SBE13	Antiproliferative activity against human HeLa cells assessed as growth inhibition after 24 to 72 hrs Antiproliferative activity against human HeLa cells assessed as growth inhibition after 24 to 72 hrs	[PMID: 25304894]
HeLa	EC₅₀	18 μM Compound: 38; SBE13	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
HT-29	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
LN-229	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
MCF7	EC₅₀	12 μM Compound: 38; SBE13	Antiproliferative activity against human MCF7 cells assessed as reduction in cell proliferation incubated for 72 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell proliferation incubated for 72 hrs	[PMID: 35878418]
PC-3	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
SK-OV-3	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
SKW 6.4	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human SKW 6.4 cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human SKW 6.4 cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]
U2OS	EC₅₀	5 μM Compound: 38; SBE13	Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs	[PMID: 35878418]

In Vitro

SBE13 significantly reduce cell proliferation and induce apoptosis in HeLa cells, with an EC₅₀ of 18 μM^[1]. SBE13 (1-100 μM) shows no effect on Caspase 3/7 activity in NIH-3T3 cells. SBE13 (66 and 100 μM) does not change morphology after treatment of primary cells. SBE13 (10 and 100 μM) reduces pRb staining in primary cells, and this indicates a G0/G1 arrest^[2]. SBE13 (66 and 100 μM) increases levels of cyclin B1, phospho histone H3, Wee1, Emi1 and securin, and results in cleavage of Cdc27 in HeLa cells. SBE13 (10 and 100 μM) also induces apoptosis of HeLa cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 775294-82-1
Molecular Weight 442.94
Formula C₂₄H₂₇ClN₂O₄
SMILES

COC1=CC=C(CCNCC2=CC=C(OCC3=CC=C(Cl)N=C3)C(OC)=C2)C=C1OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Keppner S, et al. Identification and validation of a potent type II inhibitor of inactive polo-like kinase 1. ChemMedChem. 2009 Nov;4(11):1806-9. [Content Brief]

[2]. Keppner S, et al. Fate of primary cells at the G?/S boundary after polo-like kinase 1 inhibition by SBE13. Cell Cycle. 2011 Feb 15;10(4):708-20. Epub 2011 Feb 15. [Content Brief]

[3]. Keppner S, et al. Biological impact of freezing Plk1 in its inactive conformation in cancer cells. Cell Cycle. 2010 Feb 15;9(4):761-73. Epub 2010 Feb 16. [Content Brief]

Calculators

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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