Tetracaine hydrochloride
Based on 1 publication(s) in Google Scholar
Tetracaine hydrochloride (Amethocaine hydrochloride) is a calcium channel protein inhibitor and blocks voltage-sensitive release of Ca2+ from sarcoplasmic reticulum. Tetracaine hydrochloride is mainly used topically in ophthalmology and as an antipruritic.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 136-47-0
- Formula: C15H25ClN2O2
- Molecular Weight:300.82
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Storage:
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tetracaine hydrochloride
MoreAll Calcium Channel Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO-K1 | IC50 |
0.53 μM
Compound: tetracaine
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Inhibition of human NaV1.5 alpha subunit expressed in CHOK1 cells at -90 mV holding potential by patch clamp electrophysiological assay
Inhibition of human NaV1.5 alpha subunit expressed in CHOK1 cells at -90 mV holding potential by patch clamp electrophysiological assay
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[PMID: 22770500] |
| CHO-K1 | IC50 |
1.1 μM
Compound: tetracaine
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Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay
Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay
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[PMID: 22770500] |
| HEK293 | IC50 |
0.11 μM
Compound: tetracaine
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Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay
Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay
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[PMID: 22770500] |
| HEK293 | IC50 |
0.49 μM
Compound: Tetracaine
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Inhibition of recombinant human Nav1.2 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
Inhibition of recombinant human Nav1.2 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
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[PMID: 27015369] |
| HEK293 | IC50 |
0.52 μM
Compound: Tetracaine
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Inhibition of recombinant human Nav1.6 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
Inhibition of recombinant human Nav1.6 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
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[PMID: 27015369] |
| HEK293 | IC50 |
0.62 μM
Compound: tetracaine
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Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -90 mV holding potential by patch clamp electrophysiological assay
Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -90 mV holding potential by patch clamp electrophysiological assay
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[PMID: 22770500] |
| HEK293 | IC50 |
0.65 μM
Compound: Tetracaine
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Inhibition of human Nav1.5 expressed in HEK293 cells by whole cell-patch clamp method
Inhibition of human Nav1.5 expressed in HEK293 cells by whole cell-patch clamp method
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[PMID: 27015369] |
| HEK293 | IC50 |
0.74 μM
Compound: Tetracaine
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Inhibition of recombinant human Nav1.3 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
Inhibition of recombinant human Nav1.3 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
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[PMID: 27015369] |
| HEK293 | IC50 |
33 μM
Compound: tetracaine
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Inhibition of human NaV1.7 F1737A mutant expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay
Inhibition of human NaV1.7 F1737A mutant expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay
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[PMID: 22770500] |
| HEK293 | IC50 |
7.51 μM
Compound: Tetracaine
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Inhibition of human NaV1.7 expressed in HEK293 cells by [14C]guanidinium influx assay
Inhibition of human NaV1.7 expressed in HEK293 cells by [14C]guanidinium influx assay
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[PMID: 21570288] |
Tetracaine hydrochloride (Amethocaine hydrochloride) is used to alter the function of calcium release channels (ryanodine receptors) that control the release of calcium from intracellular stores. Tetracaine hydrochloride is an allosteric blocker of channel function. At low concentrations, tetracaine causes an initial inhibition of spontaneous calcium release events, while at high concentrations, tetracaine blocks release completely[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 136-47-0
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Appearance Solid
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Molecular Weight 300.82
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Formula C15H25ClN2O2
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Color White to off-white
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SMILES
O=C(C1=CC=C(NCCCC)C=C1)OCCN(C)C.Cl
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Synonyms
Amethocaine hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (1)
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Journal Impact Factor
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Most Recent
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JACC Basic Transl Sci
JP2 and JCN Crosstalk Abrogate MURF1-Mediated JCN Ubiquitination and Degradation in Cardiomyocytes. [Abstract]2026 Apr 17;11(5):101534. PMID: 42000606
Solvent & Solubility
H2O : 50 mg/mL (166.21 mM; Need ultrasonic)
DMSO : ≥ 30 mg/mL (99.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.3242 mL | 16.6212 mL | 33.2425 mL | 83.1062 mL |
| 5 mM | 0.6648 mL | 3.3242 mL | 6.6485 mL | 16.6212 mL | |
| 10 mM | 0.3324 mL | 1.6621 mL | 3.3242 mL | 8.3106 mL | |
| 15 mM | 0.2216 mL | 1.1081 mL | 2.2162 mL | 5.5404 mL | |
| 20 mM | 0.1662 mL | 0.8311 mL | 1.6621 mL | 4.1553 mL | |
| 25 mM | 0.1330 mL | 0.6648 mL | 1.3297 mL | 3.3242 mL | |
| 30 mM | 0.1108 mL | 0.5540 mL | 1.1081 mL | 2.7702 mL | |
| 40 mM | 0.0831 mL | 0.4155 mL | 0.8311 mL | 2.0777 mL | |
| 50 mM | 0.0665 mL | 0.3324 mL | 0.6648 mL | 1.6621 mL | |
| 60 mM | 0.0554 mL | 0.2770 mL | 0.5540 mL | 1.3851 mL | |
| 80 mM | 0.0416 mL | 0.2078 mL | 0.4155 mL | 1.0388 mL | |
| H2O | 100 mM | 0.0332 mL | 0.1662 mL | 0.3324 mL | 0.8311 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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