Fosribnicotinamide (Synonyms: β-Nicotinamide mononucleotide; β-NM; NMN)

Name: Fosribnicotinamide
Brand: MedChemExpress
SKU: HY-F0004
Rating: 5.0 (27 reviews)

Cat. No.: HY-F0004 Purity: 99.94%: Data Sheet
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Fosribnicotinamide (β-nicotinamide mononucleotide) is a product of the nicotinamide phosphoribosyltransferase (NAMPT) reaction and a key NAD⁺ intermediate. The pharmacological activities of Fosribnicotinamide include its role in cellular biochemical functions, cardioprotection, diabetes, Alzheimer's disease, and complications associated with obesity.

For research use only. We do not sell to patients.

CAS No. : 1094-61-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 27 publication(s) in Google Scholar

Other Forms of Fosribnicotinamide:

Fosribnicotinamide-d₄ In-stock
Fosribnicotinamide (Standard) In-stock

27 Publications Citing Use of MCE Fosribnicotinamide

Bio/Physico-chemical Assay

In Vivo Efficacy Study

Flow Cytometry

: Fosribnicotinamide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Apr 28;14(7):2794-2815. [Abstract]; RAW264.7 cells were treated with FK866 (1 nM) and β-NMN (100 μΜ) 30 min before the addition of TGF-β1 (20 ng/mL) for 24 h，ELISA assay of NAMPT releasing in the culture supernatant (left), Quantification of the intracellular NAD level using a commercial kit (right).

: Fosribnicotinamide purchased from MedChemExpress. Usage Cited in: Cell Metab. 2021 Jan 5;33(1):110-127.e5. [Abstract]; Tumor growth curves and tumor burdens in C57BL/6 mice injected subcutaneously with sgCtrl or sgNampt liver cancer cells (Hepa1–6) for 3 weeks (n = 6) and C57BL/6 mice injected subcutaneously with Hepa1–6 cells with solvent control (Ctrl) or NMN (Fosribnicotinamide;300 mg/kg body weight; daily;intraperitoneal injection for 2 weeks beginning on day 7 after LLC cells, Pan02 cells, or Hepa1-6-CD38 cells were subcutaneously implanted).

: Fosribnicotinamide purchased from MedChemExpress. Usage Cited in: Cell Metab. 2021 Jan 5;33(1):110-127.e5. [Abstract]; Representative flow staining indicated cells in tumor xenograft of indicated groups.(NMN, Fosribnicotinamide;300 mg/kg body weight; daily;intraperitoneal injection for 2 weeks beginning on day 7 after LLC cells, Pan02 cells, or Hepa1-6-CD38 cells were subcutaneously implanted)

: Fosribnicotinamide purchased from MedChemExpress. Usage Cited in: Cell Metab. 2021 Jan 5;33(1):110-127.e5. [Abstract]; Representative flow staining (left panel) and frequency (right panel) of CD8+ T cells and PD-1+ CD8+ T cells in tumor xenograft of indicated groups ((NMN, Fosribnicotinamide;300 mg/kg body weight; daily,ip; PD-L1 antibodies (100 μg of anti-PD-L1 per mouse; twice a week) or combination via intraperitoneal injection for 2 weeks beginning on day 7 after LLC cells, Pan02 cells, or Hepa1-6-CD38 cells were subcutaneously implanted).

: Fosribnicotinamide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19626-19634. [Abstract]; p53−/− mice were treated per divided lowdose Dox protocol and with either vehicle control or NMN (Fosribnicotinamide; 500 mg/kg, i.p., 3 times per week on alternating days) over the duration of the entire study. Unless indicated, all analyses were performed at study week 10. LV ejection fraction (LVEF) of p53−/−mice treated with Dox only or Dox plus NMN was measured by echocardiogram at study week 7 and 10 (n = 8). Mitochondrial respiration and ATP levels were measured in cardiac tissue (n ≥ 3).

: Fosribnicotinamide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19626-19634. [Abstract]; p53−/− mice were treated per divided lowdose Dox protocol and with either vehicle control or NMN (Fosribnicotinamide; 500 mg/kg, i.p., 3 times per week on alternating days) over the duration of the entire study. Unless indicated, all analyses were performed at study week 10. Immunoblot of representative mtDNA-encoded protein MT-CO1 in cardiac tissue. GAPDH is shown as protein loading control.

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Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Fosribnicotinamide (β-nicotinamide mononucleotide) has several beneficial pharmacological activities. Mostly mediated by its involvement in NAD⁺ biosynthesis, the pharmacological activities of NMN include its role in cellular biochemical functions, cardioprotection, diabetes, Alzheimer's disease, and complications associated with obesity^[1].
The intracellular NAD⁺ levels are significantly decreased by knockdown or knockout of Nampt (Nampt KD or Nampt KO) or treatment with Nampt inhibitor FK866, whereas NAD⁺ levels are dramatically increased by supplement of NAD⁺ precursors NAM or NMN (0.5-1 mM). NAD⁺ precursor NMN treatment inhibited CD8⁺ T cells activation and function^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Fosribnicotinamide (β-nicotinamide mononucleotide) (500 mg/kg; i.p.; 3 times per week for 7-10 week) prevents mtDNA damage and Dox-induced cardiac dysfunction^[3].
Nampt KO markedly inhibits tumor progression, whereas Nampt metabolite Fosribnicotinamide (300 mg/kg body weight; i.p.; once every two days for 2 weeks) significantly promotes tumor growth in C57BL/6 mice (bearing wildtype Hepa1-6 cells). The reduction and increase in NAD⁺ level of respective Nampt KO and Fosribnicotinamide-treated tumors are confirmed^[2].
Fosribnicotinamide ameliorates glucose intolerance by restoring NAD⁺ levels in HFD-induced T2D mice.Fosribnicotinamide also enhances hepatic insulin sensitivity and restores gene expression related to oxidative stress, inflammatory response, and circadian rhythm, partly through SIRT1 activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL6 mice (p53^−/− mice)^[3]
Dosage:	500 mg/kg
Administration:	I.p.; 3 times per week for 7-10 week
Result:	Prevented the significant decline in cardiac function of Dox-treated p53^−/− mice (study week 7 versus 10) along with rescuing the decreased mitochondrial respiration and tissue ATP depletion caused by Doxorubicin (Dox).

Molecular Weight

334.22

Formula

C₁₁H₁₅N₂O₈P

CAS No.

1094-61-7

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@H](O)[C@@H](COP(O)([O-])=O)O[C@H]1[N+]2=CC=CC(C(N)=O)=C2

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 83.33 mg/mL (249.33 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9920 mL	14.9602 mL	29.9204 mL
5 mM	0.5984 mL	2.9920 mL	5.9841 mL
10 mM	0.2992 mL	1.4960 mL	2.9920 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (299.20 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

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Data Sheet (276 KB) SDS (394 KB)

COA (248 KB) HNMR (265 KB) RP-HPLC (205 KB) MS (67 KB)

Handling Instructions (2659 KB)

References

[1]. Poddar SK, et al. Nicotinamide Mononucleotide: Exploration of Diverse Therapeutic Applications of a Potential Molecule. Biomolecules. 2019;9(1):34. Published 2019 Jan 21. [Content Brief]

[2]. Lv H, et al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion [published online ahead of print, 2020 Nov 3]. Cell Metab. 2020;S1550-4131(20)30554-4. [Content Brief]

[3]. Li J, et al. p53 prevents doxorubicin cardiotoxicity independently of its prototypical tumor suppressor activities. Proc Natl Acad Sci U S A. 2019;116(39):19626-19634. [Content Brief]

[4]. Yoshino J, et al Nicotinamide mononucleotide, a key NAD(+) intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice. Cell Metab. 2011;14(4):528-536. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.9920 mL	14.9602 mL	29.9204 mL	74.8010 mL
	5 mM	0.5984 mL	2.9920 mL	5.9841 mL	14.9602 mL
	10 mM	0.2992 mL	1.4960 mL	2.9920 mL	7.4801 mL
	15 mM	0.1995 mL	0.9973 mL	1.9947 mL	4.9867 mL
	20 mM	0.1496 mL	0.7480 mL	1.4960 mL	3.7401 mL
	25 mM	0.1197 mL	0.5984 mL	1.1968 mL	2.9920 mL
	30 mM	0.0997 mL	0.4987 mL	0.9973 mL	2.4934 mL
	40 mM	0.0748 mL	0.3740 mL	0.7480 mL	1.8700 mL
	50 mM	0.0598 mL	0.2992 mL	0.5984 mL	1.4960 mL
	60 mM	0.0499 mL	0.2493 mL	0.4987 mL	1.2467 mL
	80 mM	0.0374 mL	0.1870 mL	0.3740 mL	0.9350 mL
	100 mM	0.0299 mL	0.1496 mL	0.2992 mL	0.7480 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Fosribnicotinamide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fosribnicotinamide1094-61-7β-Nicotinamide mononucleotide β-NM NMNEndogenous MetabolitecardioprotectiondiabetesAlzheimer'sdiseaseobesitycancerNADintermediateInhibitorinhibitorinhibit

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Fosribnicotinamide (Synonyms: β-Nicotinamide mononucleotide; β-NM; NMN)

Customer Review

Other Forms of Fosribnicotinamide:

Fosribnicotinamide Related Antibodies

27 Publications Citing Use of MCE Fosribnicotinamide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Fosribnicotinamide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Fosribnicotinamide Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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