AChE/BChE-IN-42
AChE/BChE-IN-42 is a potent competitive inhibitor of AChE and BChE, with Ki values of 24.27 nM and 81.89 nM, respectively. AChE/BChE-IN-42 binds tightly to the active sites (CAS and PAS) of AChE and BChE through multiple strong interactions, thereby competitively inhibiting the activity of both cholinesterases. AChE/BChE-IN-42 can be used for research on Alzheimer's disease.
For research use only. We do not sell to patients.
- Formula: C28H29F3N4O4
- Molecular Weight:542.55
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
AChE 24.27 nM (Ki) |
BChE 81.89 nM (Ki) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| SH-SY5Y | IC50 |
37.32 μM
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Cytotoxicity against human neuroblastoma SH-SY5Y cells assessed as reduction in cell viability incubated for 24 hrs by crystal violet staining method.
Cytotoxicity against human neuroblastoma SH-SY5Y cells assessed as reduction in cell viability incubated for 24 hrs by crystal violet staining method.
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42585890 |
In Vitro
AChE-IN-121 (200-3.125 μM; 24 h) shows low cytotoxicity against human neuroblastoma SH-SY5Y cells with an IC50 of 37.32 μM[1].
AChE-IN-121 is a potent competitive inhibitor of Electrophorus electricus AChE with a Ki of 24.27 nM and demonstrates selectivity for AChE over BChE[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SH-SY5Y
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Concentration:3.125, 6.25, 12.5, 25, 50, 100, 200 μM
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Incubation Time:24 h
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Result:Exhibited low cytotoxicity toward SH-SY5Y cells with an IC50 value of 37.32 μM.
Chemical Information
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Molecular Weight 542.55
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Formula C28H29F3N4O4
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SMILES
COC1=CC(/C=N/NC(C2=CC=C(C=C2)OC3=NC=CC=C3C(F)(F)F)=O)=CC(CN4CC(C)CCC4)=C1O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Carbohydrates and Mucins: Periodic Acid-Schiff (PAS) Staining
Periodic acid-Schiff staining detects tissue carbohydrates and mucosubstances by oxidizing carbohydrate glycol groups with periodic acid to generate aldehydes, which then react with Schiff reagent to produce a magenta reaction product; classic reports established the method for mucin and polysaccharide-containing structures in fixed tissue sections. PAS staining can demonstrate neutral mucins and goblet-cell mucin, but it is not specific for mucin because glycogen and other PAS-positive tissue components can also stain; diastase/PAS-D is used when glycogen removal is needed to distinguish glycogen-dependent PAS signal from non-glycogen PAS-positive mucosubstances.
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AChE/BChE-IN-42 Related Classifications
HY-189407 Related Classifications
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