Adenosine 3′-monophosphate
Based on 1 Customer Validation
Adenosine 3’-monophosphate (3’-AMP) is a nucleotide. Adenosine 3’-monophosphate is a cyclic AMP production agonist. Adenosine 3’-monophosphate increases cyclic AMP levels concentration-dependently in NG108-15 cells. Adenosine 3’-monophosphate can be used in the determination of acid phosphatase activity of human serum, which is estabilished as a laboratory procedure in the diagnosis of prostate cancer, metastatic mammary cancer and Gaucher’s disease. Adenosine 3’-monophosphate inhibits human aortic and coronary vascular smooth muscle cell proliferation via A2B receptors.
For research use only. We do not sell to patients.
- Purity: 99%
- CAS No.: 84-21-9
- Formula: C10H14N5O7P
- Molecular Weight:347.22
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | EC50 |
0.44 μM
Compound: 3'-AMP
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Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay
Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay
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[PMID: 22738238] |
Adenosine 3’-monophosphate (Compound A3P) induces cyclic AMP concentration-dependently in NG108-15 cells(IC50 = 190 μM)[1].
Adenosine 3’-monophosphate (Compound 3’-AMP) (0-10 μmol/L) increases adenosine levels in rat aortic vascular smooth muscle cells (AVSMCs)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat and human aortic vascular smooth muscle cells
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Concentration:0, 3, 10 μmol/L
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Incubation Time:Every 24 h for 4 days
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Result:Significantly inhibited proliferation (cell number) of both human and rat AVSMCs.
Chemical Information
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CAS No. 84-21-9
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Appearance Solid
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Molecular Weight 347.22
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Formula C10H14N5O7P
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Color White to off-white
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SMILES
NC1=C2C(N([C@@H]3O[C@H](CO)[C@@H](OP(O)(O)=O)[C@H]3O)C=N2)=NC=N1
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Synonyms
3′-AMP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 0.5 mg/mL (1.44 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. S Ohkubo, et al. Effects of AMP derivatives on cyclic AMP levels in NG108-15 cells. Br J Pharmacol. 2000 Mar;129(6):1244-50. [Content Brief]
[2]. Ellis, G., et al., (1971). Colorimetric determination of serum acid phosphatase activity using adenosine 3'-monophosphate as substrate. Journal of clinical pathology, 24(6), 493–500. [Content Brief]
[3]. Jackson, E. K., et al., (2011). 2',3'-cAMP, 3'-AMP, and 2'-AMP inhibit human aortic and coronary vascular smooth muscle cell proliferation via A2B receptors. American journal of physiology. Heart and circulatory physiology, 301(2), H391–H401. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.8800 mL | 14.4001 mL | 28.8002 mL | 72.0005 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Adenosine 3′-monophosphate Related Classifications
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