Adenosine 5'-O-(3-thiotriphosphate)

ATPγS (0.2-2 mM; 0-180 min) utilization efficiency is much less than that of ATP in the Abl tyrosine kinase reaction with Abltide-GST, with a steady-state thiophosphorylation ratio of about 14%^[1].
Adenosine 5'-O-(3-thiotriphosphate) (0.2 mM; 0-60 min) shows differences in utilization efficiency compared to ATP in the ERK2 reaction with MBP, with a thiophosphorylated MBP ratio of approximately 35% at 60 min^[1].
ATPγS enables Phos-tag affinity electrophoresis detection of GST-cJun thiophosphorylation by JNK1, demonstrating its capability to capture the thiophosphate moiety of a serine residue^[1].
ATPγS (1 mM; 0-240 min; 1-3 days) is accepted as readily as ATP by PhoR in the autophosphorylation reaction, with a steady-state thiophosphorylation ratio of about 48%^[1].
ATPγS (1 μM-10 mM; 3 min preincubation and 20 min reaction) inhibits purified C1·C2·forskolin adenylyl cyclase with a K_i of 170 μM in the presence of Mn²⁺ and 1900 μM in the presence of Mg²⁺, demonstrating only little affinity gain over ATP under Mn²⁺ conditions and a substantial loss of affinity under Mg²⁺ conditions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .