alpha-1,3-Fucosyltransferase (α1,3FucT)

Product Information
This product is recombinantly expressed in E. coli, with a C-terminal His Tag, a molecular weight of 37 kDa, and a storage buffer component of 20 mM Tris-HCl, pH 8.0, 10% glycerol. It can be used to connect fucose to the sugar chain of Galβ1-3GlcOR in an α1,3 connection manner, and is a key enzyme that catalyzes the synthesis of 3'-fucosyllactose.

Instructions
1. Add substrate according to the reaction system, 10 mM MnCl₂, and appropriate buffer salts (such as Tris-HCl) can be added to adjust the pH to 7.5, and add appropriate amount of enzyme (according to reaction requirements);
2. After mixing, incubate the sample at 37°C for 2-4h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.