Anti-CD71/TfR1 Antibody
Based on 1 Customer Validation
Anti-CD71/TfR1 Antibody (JR-141 antibody (uncoupled from iduronate 2-sulfatase)) is a humanized anti-human transferrin receptor (CD71/TfR1) antibody. Anti-CD71/TfR1 Antibody is the antibody part of the fusion protein Pabinafusp alfa (HY-P99797). The recommend isotype control of Anti-CD71/TfR1 Antibody: Human IgG1 kappa, Isotype Control (HY-P99001).
For research use only. We do not sell to patients.
- Purity: ≥99.0%
- Molecular Weight:146.3 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Human IgG1 kappa
Human
Pabinafusp alfa (HY-P99797) is a blood-brain barrier-penetrating fusion protein that inhibits the deposition of heparan sulfate in the central nervous system of mice with mucopolysaccharidosis II, improves learning ability in the mice, and prevents neuronal damage in the central nervous system[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
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Immobilized Human Transferrin R/CD71 Protein, His Tag can bind Anti-CD71/TfR1 Antibody. The EC50 for this effect is 3.461 ng/mL. -
Flow cytometric analysis of 1X106 Juakat cells with Anti-CD71 Antibody (HY-P991047, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 146.3 kDa
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Color Colorless to light yellow
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SMILES
[Anti-CD71/TfR1 Antibody]
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Synonyms
JR-141 antibody (uncoupled from iduronate 2-sulfatase)
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (256 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yamamoto R, et al. Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II. Mol Genet Metab Rep. 2021 Apr 18;27:100758. [Content Brief]
[2]. Sonoda H, et al. A Blood-Brain-Barrier-Penetrating Anti-human Transferrin Receptor Antibody Fusion Protein for Neuronopathic Mucopolysaccharidosis II. Mol Ther. 2018;26(5):1366-1374. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)