Anti-CD71/TfR1 Antibody  (Synonyms: JR-141 antibody (uncoupled from iduronate 2-sulfatase))

Anti-CD71/TfR1 Antibody (JR-141 antibody (uncoupled from iduronate 2-sulfatase)) is a humanized anti-human transferrin receptor (CD71/TfR1) antibody. Anti-CD71/TfR1 Antibody is the antibody part of the fusion protein Pabinafusp alfa (HY-P99797). The recommend isotype control of Anti-CD71/TfR1 Antibody: Human IgG1 kappa, Isotype Control (HY-P99001)^[1][2].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Pabinafusp alfa (HY-P99797) is a blood-brain barrier-penetrating fusion protein that inhibits the deposition of heparan sulfate in the central nervous system of mice with mucopolysaccharidosis II, improves learning ability in the mice, and prevents neuronal damage in the central nervous system^[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

146.3 kDa

Liquid

Colorless to light yellow

[Anti-CD71/TfR1 Antibody]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Immobilized Human Transferrin R/CD71 Protein, His Tag can bind Anti-CD71/TfR1 Antibody. The EC₅₀ for this effect is 3.461 ng/mL.
• 
  Flow cytometric analysis of 1X10⁶ Juakat cells with Anti-CD71 Antibody (HY-P991047, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Yamamoto R, et al. Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II. Mol Genet Metab Rep. 2021 Apr 18;27:100758.  [Content Brief]

  [2]. Sonoda H, et al. A Blood-Brain-Barrier-Penetrating Anti-human Transferrin Receptor Antibody Fusion Protein for Neuronopathic Mucopolysaccharidosis II. Mol Ther. 2018;26(5):1366-1374.  [Content Brief]