Anti-GPC3 Antibody (YP7)
Based on 1 Customer Validation
Anti-GPC3 Antibody (YP7) is a kind of mouse IgG1 κ chimeric antibody, targeting to human GPC3. Anti-GPC3 Antibody (YP7) reacts with the C-lobe of cell surface-associated glypican-3 (GPC3). Anti-GPC3 Antibody (YP7) can be used for the research of cancer, such as hepatocellular carcinoma (HCC).
For research use only. We do not sell to patients.
- Purity: ≥95.0%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Mouse IgG1 kappa
Human
GPC3
Anti-GPC3 Antibody (YP7) (1 μg/mL) has excellent binding affinity and specificity for the native form of GPC3 in hepatocellular carcinoma (HCC) and other GPC3-expressing cancers[1].
Anti-GPC3 Antibody (YP7) (IR700-YP7, IR700 and YP7 conjugation) (10 μg/mL, 6 h) induces lysosome swelling, budding and rupture in A431/G1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anti-GPC3 Antibody (YP7) (IR700-YP7, IR700 and YP7 conjugation) (100 μg, i.v.) inhibits tumor growth companied with NIR light in A431/G1 tumor mice (homozygote athymic nude mice)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HepG2 tumor mice models (4-6 weeks, female, BALB/c nu/nu mice)[1]
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Dosage:5 mg/kg
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Administration:Intravenously injection, twice a week for 2 weeks
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Result:Inhibited tumor growth.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vitro photoimmunotherapy; in vivo anti-tumor activity; in vivo imaging; in vivo photoimmunothe
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Flow cytometric analysis of 1X106 Jurkat cells labeling Anti-GPC3 Antibody (HY-P99840, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-GPC3 Antibody (YP7)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Phung Y, et al. High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening. MAbs. 2012 Sep-Oct;4(5):592-9. [Content Brief]
[2]. Hanaoka H, et al. Photoimmunotherapy of hepatocellular carcinoma-targeting Glypican-3 combined with nanosized albumin-bound paclitaxel. Nanomedicine (Lond). 2015;10(7):1139-47. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-GPC3 Antibody (YP7) Related Classifications
HY-P990840 Related Classifications
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