Anti-Mouse CD71/TfR1 Antibody (8D3)
Based on 1 Customer Validation
Anti-Mouse CD71/TfR1 Antibody (8D3) is a rat-derived anti-mouse CD71/TfR1 IgG2a type antibody inhibitor. Anti-Mouse CD71/TfR1 Antibody (8D3) depletes of CD71+ erythroid cells but does not change percentages of Tregs in neonatal mice. Anti-Mouse CD71/TfR1 Antibody (8D3) enhances neonatal mice defence against infection.
For research use only. We do not sell to patients.
- Purity: 98.77%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
CD71/TfR1
In Vitro
The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse CD71/TfR1 Antibody (8D3) (150 μg, i.p., on days 9 after birth) depletes of CD71+ erythroid cells but does not change percentages of Tregs in neonatal mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:8-day-old neonatal mice [1]
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Dosage:150 μg
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Administration:Intraperitoneally injection at days 5 and 6 after birth
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Result:Significant reduced the number of recoverable L. monocytogenes bacteria.
Significantly produced more TNF-α and upregulated expression of the co-stimulatorymolecules CD40, CD80 and CD86.
Restored neonatal resistance to E. coli infection.
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Animal Model:Neonatal mice [2]
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Dosage:150 μg
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Administration:Intraperitoneally injection at day 9 after birth
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Result:Reduced approximately 60% of CD71+ erythroid cells in the spleen of mice.
Had no significant effects on the percentage of Tregs.
Significantly up-regulated VISTA expression in both the percentages and MFI of VISTA expression.
Upregulated Id1 and Id2 genes and produced TGF-β.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vivo depletion of CD71+ cells; Immunofluorescence; Immunohistochemistry (frozen); Western blot
Verified Bioactivity
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Flow cytometric analysis of 1X106 MC38 cells with Anti-Mouse CD71/TfR1 Antibody (8D3) (HY-P990129, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rat IgG H&L (HY-P89199) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2a kappa (HY-P990679, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Immobilized TFRC Protein, Mouse (HEK293, His, HY-P72455) can bind Anti-Mouse CD71/TfR1 Antibody (8D3). The EC50 for this effect is 10.58 ng/mL. -
Loaded Anti-Mouse CD71/TfR1 Antibody (8D3) on CM5 biosensor, can bind TFRC Protein, Mouse (HEK293, His, HY-P72455) with an affinity constant of 1.80E-09 M as determined in SPR assay.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD71/TfR1 Antibody (8D3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Goulet DR, Atkins WM. Considerations for the Design of Antibody-Based Therapeutics. J Pharm Sci. 2020 Jan;109(1):74-103. [Content Brief]
[2]. Elahi S, et al. Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection. Nature. 2013 Dec 5;504(7478):158-62. [Content Brief]
[3]. Shahbaz S, et al. CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β. PLoS Biol. 2018 Dec 14;16(12):e2006649. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD71/TfR1 Antibody (8D3) Related Classifications
HY-P990129 Related Classifications
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