Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2)

Cat. No.: HY-P990144 Purity: 95.00%: Data Sheet
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Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) is an anti-mouse/human VLA-4/CD49d IgG2b monoclonal antibody. Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) can reduce VLA-4 positive cells. Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) can inhibit the migration of Tc1 cells to tumors. Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) inhibits the retention of leukemia cells in the spleen and bone marrow by blocking VLA-4. Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) can be used for research on inflammation conditions and cancer such as experimental autoimmune encephalomyelitis (EAE), melanoma and leukemia.

For research use only. We do not sell to patients.

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) Featured Recommendations:

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

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αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Isotype

Rat IgG2b kappa

Recommend Isotype Controls

Rat IgG2b kappa, Isotype Control

Species Reactivity

Human/Mouse

IC₅₀ & Target^[1]^[2]^[3]

α4β1

In Vitro

Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) (20 μg/mL, 15 min) significantly inhibits the adhesion of Tc1 cells to VCAM-1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) (0.1 mg, i.p., once every other day, from day 4 to day 14) significantly reduces the incidence of diseases in female BALB/c mice with a virus induced experimental autoimmune encephalomyelitis (EAE) model^[1].
Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) (150 μg, i.v., single dose) significantly inhibits the migration of Tc1 cells to intracranial tumors in C57BL/6 mice bearing M05 tumors^[2].
Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) (10 mg/kg, i.p., twice a week, for 5 weeks) significantly reduces tumor cells in the spleen and bone marrow in wild type C57BL/6J mice with a chronic lymphocytic leukemia (CLL) model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	1×10⁶ PFU SFV-A7 virus induced female BALB/c mice (6-8 weeks)^[1]
Dosage:	0.1 mg
Administration:	Intraperitoneal injection (i.p.), once every other day, from day 4 to day 14
Result:	Significantly reduced the incidence rate to 10 % and the average incidence day to 14.0 days. Significantly reduced inflammation and some mouse brain tissues were completely free of inflammation. Reduced VCAM-1 expression and decreased VLA-4 positive cells.

Animal Model:	5×10⁴ M05 cells injected C57BL/6 mice (5-9 weeks)^[2]
Dosage:	150 μg
Administration:	Intravenous injection (i.v.), single dose
Result:	Significantly inhibited the migration of Tc1 cells (inhibition of 85 %).

Animal Model:	5×10⁶ splenocytes from TCL1-tg mouse injected wild type C57BL/6J mice^[3]
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection (i.p.), twice a week, for 5 weeks
Result:	Reduced the number of tumor cells in the spleen and bone marrow. Reduced peripheral blood leukemia cells.

Gene ID

16401 [NCBI]

Accession

Q00651

Application

in vivo VLA-4 neutralization; in vitro VLA-4 neutralization; Flow cytometry

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2)]

Formulation

Please refer to the lot-specific COA for specific buffer information.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Biological Activity

Flow cytometric analysis of 1×10⁶ Jurkat cells labelling VLA-4 (red) with Anti-Mouse/Human VLA-4 Antibody (HY-P990144). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2b kappa Isotype Control (HY-P990682, blue) was used as the isotype control.

Purity & Documentation

Purity: 95.00%

Select Batch:

Data Sheet (262 KB) SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. Soilu-Hänninen M, et al. Therapy with antibody against leukocyte integrin VLA-4 (CD49d) is effective and safe in virus-facilitated experimental allergic encephalomyelitis. J Neuroimmunol. 1997 Jan;72(1):95-105. [Content Brief]

[2]. Sasaki K, et al. Preferential expression of very late antigen-4 on type 1 CTL cells plays a critical role in trafficking into central nervous system tumors. Cancer Res. 2007 Jul 1;67(13):6451-8. [Content Brief]

[3]. Szenes E, et al. TCL1 transgenic mice as a model for CD49d-high chronic lymphocytic leukemia. Leukemia. 2020 Sep;34(9):2498-2502. [Content Brief]

Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse/Human VLA-4/CD49d Antibody (PS/2)IntegrinVLA-4/CD49dα4β1EAEM05leukemiamelanomaTc1Inhibitorinhibitorinhibit

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