Anti-Rat CD28 Antibody (JJ316)
Anti-Rat CD28 Antibody (JJ316) is a mouse-derived agonistic IgG1 κ type antibody, targeting to rat CD28. Anti-Rat CD28 Antibody (JJ316) can induce T cell proliferation. Anti-Rat CD28 Antibody (JJ316) can be used for the researches of inflammation, immunology and neurological disease, such as experimental autoimmune encephalomyelitis (EAE) and graft-versus-host disease (GvHD).
For research use only. We do not sell to patients.
- Molecular Weight:150 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Rat
IC50 & Target
CD28
In Vivo
Anti-Rat CD28 Antibody (JJ316) (0.1-1 mg/rat, i.v., a single dose) induces regulator T cell (Treg) proliferation in Adult Lewis rats[1].
Anti-Rat CD28 Antibody (JJ316) (0.03-1 mg/rat, i.v., a single dose) Reduced the severity of EAE in myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) rat models[1].
Anti-Rat CD28 Antibody (JJ316) (1 mg/rat, i.v., a single dose) increase CD4+CD25+ T cells and increases IL-2, IL-4, IL-10 and Foxp3 levels in Lewis rats[2].
Anti-Rat CD28 Antibody (JJ316) (1 mg/rat, i.v., a single dose) suppresses graft-versus-host disease (GvHD) reactions in spleen transferred Lewis × DA hybrid rats[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Adult Lewis rats[1]
-
Dosage:0.1, 0.3 and 1 mg/rat
-
Administration:Intravenously injection
-
Result:Led to a significant increase in CD25+ cells among CD4+ cells in peripheral lymph nodes and in the spleen.
Led to a increase in CD25+ cells among CD4- cells at 1 mg/rat.
Increased transferred Treg cells further up-regulated CD25 expression.
Showed 97% of Tconv cells undergone at least one cell division at 1 mg/rat.
Showed that the vast majority of CD4+CD25+ cells, and all CD4+CD25high cells, continued to express FoxP3.
-
Animal Model:Myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) rat models[1]
-
Dosage:0.03, 0.1, 0.3 and 1 mg/rat
-
Administration:Intravenously injection, at days 0 and 10
-
Result:Immediately ameliorated disease activity at 1 mg/rat.
Reduced the severity of EAE.
-
Animal Model:Spleen transferred Lewis × DA hybrid rats[2]
-
Dosage:1 mg/rat
-
Administration:Intravenously injection
-
Result:Showed no enlargement was found in the PLN on the left side.
Inhibited the GvHD mortality significantly.
Showed relatively few infiltrating mononu-clear cells and almost normal tissue architecture.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
-
Product Image
Application
in vivo T cell stimulation/activation
Chemical Information
-
Molecular Weight 150 kDa
-
SMILES
[Anti-Rat CD28 Antibody (JJ316)]
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Beyersdorf N, et al. Selective targeting of regulatory T cells with CD28 superagonists allows effective therapy of experimental autoimmune encephalomyelitis. J Exp Med. 2005 Aug 1;202(3):445-55. [Content Brief]
[2]. Kitazawa Y, et al. Superagonist CD28 antibody preferentially expanded Foxp3-expressing nTreg cells and prevented graft-versus-host diseases. Cell Transplant. 2009;18(5):627-37. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Rat CD28 Antibody (JJ316) Related Classifications
HY-P990819 Related Classifications
Powered by Bioz