Copy Link Print Information

MRP4 Antibody (YA3530)

(Synonyms: MRP4; MOATB; MOAT-B; EST170205; ABCC 4 antibody; ABCC4 antibody; ATP binding cassette sub family C (CFTR/MRP) member 4 antibody; ATP binding cassette sub family C member 4 antibody)
Customer Review

Based on 1 Customer Validation

MRP4 Antibody (YA3530) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MRP4 Antibody (YA3530)
  • MRP4 Antibody (YA3530) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MRP4 Antibody (YA3530) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 150 kDa
Immunogen

Purified recombinant fragment of human ABCC4 (AA: 631-692) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MRP4 Antibody (YA3530)
    Flow cytometric analysis of 1X10^6 A549 cells labeling ABCC4 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    MRP4 is an ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins. Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4. Mediates the cotransport of bile acids with reduced glutathione (GSH). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules. Confers resistance to anticancer agents such as methotrexate[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Basolateral cell membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is widely expressed, with particularly high levels in the prostate, but is almost undetectable in the liver. (Hepatocyte sinusoidal membrane)

  • Isoforms & Post-Translational Modification

    O15439 has 4 isomers: O15439-1: 149527 Da (predicted); O15439-2: 144201 Da (predicted); O15439-3: 96841 Da (predicted); O15439-4: 88138 Da (predicted).
    N-glycosylated; leading to substrate-selective effects on its transport activity

  • Subunit

    Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); this interaction accelerates MRP4 internalization

  • SwissProt ID

    O15439

  • Gene ID
    10257 [NCBI]
  • Synonyms

    MRP4; MOATB; MOAT-B; EST170205; ABCC 4 antibody; ABCC4 antibody; ATP binding cassette sub family C (CFTR/MRP) member 4 antibody; ATP binding cassette sub family C member 4 antibody

MRP4 Antibody (YA3530) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling ABCC4 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • MRP4 Antibody (YA3530) Product Image
    1/16
  • Experimental Validation Results for MRP4 Antibody (YA3530)
  • MRP4 Antibody (YA3530) Product Image
  • Experimental Validation Results for MRP4 Antibody (YA3530)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling ABCC4 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for MRP4 Antibody (YA3530)
  • MRP4 Antibody (YA3530) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 A549 cells labeling ABCC4 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • MRP4 Antibody (YA3530) Product Image FC