ATG2A Antibody (YA4320)(PBS only)

Cat. No.: HY-P84623A: Data Sheet User Guide for Antibodies
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ATG2A Antibody (YA4320) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATG2A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ATG2A Antibody (YA4320) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATG2A.

Host

Mouse

Molecular Weight

Predicted band size: 212.8 kDa;

Observed band size: 215 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q2TAZ0

Gene ID

23130 [NCBI]

Immunogen

Purified recombinant fragment of human ATG2A (AA: 325-429) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Lipid transfer protein involved in autophagosome assembly (PubMed:28561066, PubMed:30952800, PubMed:31271352). Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (PubMed:30952800, PubMed:31271352). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PubMed:30952800, PubMed:31271352). Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352). Also regulates lipid droplets morphology and distribution within the cell (PubMed:22219374, PubMed:28561066)

Subcellular Localization：Preautophagosomal structure membrane; Peripheral membrane protein; Lipid droplet; Endoplasmic reticulum membrane; Peripheral membrane protein

Isoforms & Post-Translational Modification：Q2TAZ0 has 4 isomers: Q2TAZ0-1: 212860 Da (predicted); Q2TAZ0-3: 213087 Da (predicted); Q2TAZ0-4: 35255 Da (predicted); Q2TAZ0-5: 13576 Da (predicted).

Subunit：Interacts with ATG9A (via C-terminus) (PubMed:32610138, PubMed:33106659, PubMed:33850023). Interacts (via WIPI-interacting region) with WDR45B/WIPI3 (PubMed:32483132). Interacts (via WIPI-interacting region) with WDR45/WIPI4 (PubMed:28820312, PubMed:32483132). Interacts with TMEM41B (PubMed:33850023). Interacts with VMP1 (PubMed:33850023)

Synonyms

KIAA0404, ATG2A, Autophagy-related protein 2 homolog A

Documentation

User Guide for Antibodies (1077 KB)