ATPIF1 Antibody (YA4562)(PBS only)

Cat. No.: HY-P84865A: Ficha de datos User Guide for Antibodies
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ATPIF1 Antibody (YA4562) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ATPIF1.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

ATPIF1 Antibody (YA4562) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ATPIF1.

Host

Mouse

Peso molecular

Predicted band size: 12 kDa;

Observed band size: 12 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9UII2

Gene ID

93974 [NCBI]

Immunogen

Purified recombinant fragment of human ATPIF1 (AA: 1-106) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme

Subcellular Localization：Mitochondrion

Isoforms & Post-Translational Modification：Q9UII2 has 3 isomers: Q9UII2-1: 12249 Da (predicted); Q9UII2-2: 7912 Da (predicted); Q9UII2-3: 6592 Da (predicted).
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid

Subunit：Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity)

Synonyms

IP; ATPI; ATPIP

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/nci-h460-cell.html