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BRAF Antibody (YA855)

(Synonyms: BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1)
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Based on 1 Customer Validation

BRAF Antibody (YA855) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BRAF.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BRAF Antibody (YA855)
  • BRAF Antibody (YA855) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

BRAF Antibody (YA855) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BRAF.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 87 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 84 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human B Raf.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3102618

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BRAF Antibody (YA855)
    Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and HCT116 (lane 3) and 293T (lane 4) using BRAF antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Acts as a transcriptional repressor of NFATC1-mediated gene expression (By similarity)

  • Subcellular Localization

    Nucleus; Nucleus; Nucleus

  • Expression


    Tissue_specificity:It is expressed in lymphoblasts; it is weakly expressed in lymphoblasts.

  • Isoforms & Post-Translational Modification

    P26367 has 3 isomers: P26367-1: 46683 Da (predicted); P26367-2: 48218 Da (predicted); P26367-3: Da (predicted).
    Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation

  • Subunit

    Interacts with MAF and MAFB (By similarity). Interacts with TRIM11; this interaction leads to ubiquitination and proteasomal degradation, as well as inhibition of transactivation, possibly in part by preventing PAX6 binding to consensus DNA sequences (By similarity). Interacts with TLE6/GRG6 (By similarity)

  • SwissProt ID

    P15056

  • Gene ID
    673 [NCBI]
  • Synonyms

    BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

  • Research Field

    Cell Biology

BRAF Antibody (YA855) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and HCT116 (lane 3) and 293T (lane 4) using BRAF antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • BRAF Antibody (YA855) Product Image
    1/16
  • Experimental Validation Results for BRAF Antibody (YA855)
  • BRAF Antibody (YA855) Product Image
  • Experimental Validation Results for BRAF Antibody (YA855)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and HCT116 (lane 3) and 293T (lane 4) using BRAF antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for BRAF Antibody (YA855)
  • BRAF Antibody (YA855) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and HCT116 (lane 3) and 293T (lane 4) using BRAF antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • BRAF Antibody (YA855) Product Image WB