BRAF Antibody

Cat. No.: HY-P80572: FAQs
Data Sheet SDS COA User Guide for Antibodies

BRAF Antibody is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to BRAF (4E1). It can be used for WB assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	BRAF Antibody is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to BRAF (4E1). It can be used for WB assays with tag free, in the background of Human, Mouse.
Background	BRAF (4E1): This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Tag	Free
Gene ID	673
Synonyms	BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight	Predicted band size: 84 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Research Field	Cell Biology
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Host	Mouse
Reactivity	Human, Mouse, Rat
SwissProt ID	P26367
Dilution Ratio	WB: 1:500-1:1000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200
Application	WB
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG2a
Immunogen	Synthetic peptide corresponding to Human B Raf.The exact sequence is proprietary to MCE.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BRAF Antibody

Biological Activity

Technical Parameters

Properties

Documentation

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