CD235a Antibody (YA6871)
(Synonyms: Glycophorin-A; GYPA; MN sialoglycoprotein; SS-active sialoglycoprotein; PAS-2/3; Sialoglycoprotein alpha/delta; CD235a/b)CD235a Antibody (YA6871) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD235a.
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Host:
Mouse
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Isotype:
IgG
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Application:
FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:200-1:400 | 1:10000 |
Product Details
CD235a Antibody (YA6871) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD235a.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 16 kDa
Purified recombinant fragment of human CD235a (AA: 20-91) expressed in E. Coli.
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD235a (Glycophorin A, GYPA) is a highly glycosylated membrane protein found on the surface of erythrocytes and is one of the most classic markers for erythroid cells. CD235a is primarily expressed on mature erythrocytes and erythroid progenitor cells; it is used for erythrocyte identification, the study of erythroid differentiation, and research into anemia and hematological disorders.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Isoforms & Post-Translational Modification
CD235a has 3 isomers: P02724-1: 16430 Da (predicted); P02724-2: 13649 Da (predicted); P02724-3: 13000 Da (predicted).
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific) -
Subunit
Homodimer. Component of the ankyrin-1 complex in the erythrocyte, composed of ANK1, RHCE, RHAG, SLC4A1, EPB42, GYPA, GYPB and AQP1. Interacts with SLC4A1; a GYPA monomer is bound at each end of the SLC4A1 dimer forming a heterotetramer
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SwissProt ID
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Synonyms
Glycophorin-A; GYPA; MN sialoglycoprotein; SS-active sialoglycoprotein; PAS-2/3; Sialoglycoprotein alpha/delta; CD235a/b
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