Junctional Adhesion Molecule 1 Antibody (YA4597)

Cat. No.: HY-P84900: Data Sheet User Guide for Antibodies
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Junctional Adhesion Molecule 1 Antibody (YA4597) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Junctional Adhesion Molecule 1 Antibody (YA4597) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y624

Gene ID

50848 [NCBI]

Immunogen

Purified recombinant fragment of human CD321 (AA: extra 28-238) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840); (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1; (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa

Subcellular Localization：Cell junction, tight junction; Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is expressed (at the protein level) in endothelial cells, epithelial cells, and leukocytes.

Isoforms & Post-Translational Modification：Q9Y624 has 2 isomers: Q9Y624-1: 32583 Da (predicted); Q9Y624-2: 27208 Da (predicted).
N-glycosylated;(Microbial infection) Cleaved by H.pylori virulence factor PqqE. Cleavage leads to altered tight junction functions

Subunit：Interacts with the ninth PDZ domain of MPDZ (PubMed:11489913). Interacts with the first PDZ domain of PARD3 (PubMed:11489913). The association between PARD3 and PARD6B probably disrupts this interaction (By similarity). Interacts with ITGAL (via I-domain) (PubMed:15528364). Interacts with CD151 (PubMed:35067832)

Synonyms

F11R; JAM; KAT; JAM1; JAMA; JCAM; PAM-1

Documentation

User Guide for Antibodies (1077 KB)