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Junctional Adhesion Molecule 1 Antibody (YA4597)

(Synonyms: F11R; JAM; KAT; JAM1; JAMA; JCAM; PAM-1)
Customer Review

Based on 1 Customer Validation

Junctional Adhesion Molecule 1 Antibody (YA4597) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL FC ICC
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Junctional Adhesion Molecule 1 Antibody (YA4597) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Purified recombinant fragment of human CD321 (AA: extra 28-238) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
    Flow cytometric analysis of 1X10^6 HT-29 cells labeling CD321 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
    Immunofluorescence analysis of Hela cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
    Immunofluorescence analysis of NIH3T3 cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    Junctional Adhesion Molecule 1 is a Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation; (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1; (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa[1][2][3].

  • Subcellular Localization

    Cell junction, tight junction; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is expressed (at the protein level) in endothelial cells, epithelial cells, and leukocytes.

  • Isoforms & Post-Translational Modification

    Q9Y624 has 2 isomers: Q9Y624-1: 32583 Da (predicted); Q9Y624-2: 27208 Da (predicted).
    N-glycosylated;(Microbial infection) Cleaved by H.pylori virulence factor PqqE. Cleavage leads to altered tight junction functions

  • Subunit

    Interacts with the ninth PDZ domain of MPDZ (PubMed:11489913). Interacts with the first PDZ domain of PARD3 (PubMed:11489913). The association between PARD3 and PARD6B probably disrupts this interaction (By similarity). Interacts with ITGAL (via I-domain) (PubMed:15528364). Interacts with CD151 (PubMed:35067832)

  • SwissProt ID

    Q9Y624

  • Gene ID
    50848 [NCBI]
  • Synonyms

    F11R; JAM; KAT; JAM1; JAMA; JCAM; PAM-1

Junctional Adhesion Molecule 1 Antibody (YA4597) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HT-29 cells labeling CD321 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of NIH3T3 cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image
    1/16
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HT-29 cells labeling CD321 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of NIH3T3 cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA4597)
  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HT-29 cells labeling CD321 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of NIH3T3 cells labeling CD321 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD321 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image FC
  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image ICC
  • Junctional Adhesion Molecule 1 Antibody (YA4597) Product Image ICC