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CD369 Antibody (YA4466)

(Synonyms: CLEC7A; BGR; CANDF4; SCARE2; DECTIN1; CLECSF12)
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Based on 1 Customer Validation

CD369 Antibody (YA4466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD369.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD369 Antibody (YA4466)
  • Experimental Validation Results for CD369 Antibody (YA4466)
  • CD369 Antibody (YA4466) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CD369 Antibody (YA4466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD369.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Immunogen

Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD369 Antibody (YA4466)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD369 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD369 Antibody (YA4466)
    Immunofluorescence analysis of Hela cells labeling CD369 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD369 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L ( green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    CD369 is a Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans[1][2][3].

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein; Cytoplasm; Cytoplasm; Cytoplasm

  • Expression


    Tissue_specificity:Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta

    Induction:Up-regulated during differentiation from monocytes into dendritic cells

  • Isoforms & Post-Translational Modification

    Q9BXN2 has 10 isomers: Q9BXN2-1: 27627 Da (predicted); Q9BXN2-2: 22563 Da (predicted); Q9BXN2-3: 21065 Da (predicted); Q9BXN2-4: 21130 Da (predicted); Q9BXN2-5: 19218 Da (predicted); Q9BXN2-6: 14787 Da (predicted); Q9BXN2-7: 16000 Da (predicted); Q9BXN2-8: 5164 Da (predicted); Q9BXN2-9: 8363 Da (predicted); Q9BXN2-10: 13138 Da (predicted).
    Phosphorylated on tyrosine residues in response to beta-glucan binding

  • Subunit

    Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens. Interacts with CD37; this interaction controls CLEC7A-mediated IL-6 production (PubMed:17182550)

  • SwissProt ID

    Q9BXN2

  • Gene ID
    64581 [NCBI]
  • Synonyms

    CLEC7A; BGR; CANDF4; SCARE2; DECTIN1; CLECSF12

CD369 Antibody (YA4466) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD369 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CD369 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD369 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L ( green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • CD369 Antibody (YA4466) Product Image
    1/16
  • Experimental Validation Results for CD369 Antibody (YA4466)
  • Experimental Validation Results for CD369 Antibody (YA4466)
  • CD369 Antibody (YA4466) Product Image
  • Experimental Validation Results for CD369 Antibody (YA4466)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD369 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD369 Antibody (YA4466)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CD369 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD369 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L ( green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for CD369 Antibody (YA4466)
  • Experimental Validation Results for CD369 Antibody (YA4466)
  • CD369 Antibody (YA4466) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD369 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD369 Antibody (YA4466) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling CD369 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD369 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L ( green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • CD369 Antibody (YA4466) Product Image FC
  • CD369 Antibody (YA4466) Product Image ICC