CD369 Antibody (YA4466)

製品番号: HY-P84769: データシート SDS COA User Guide for Antibodies
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CD369 Antibody (YA4466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD369.

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容量	価格（税別）	在庫状況	数量
20 μL	$167	在庫あり	Estimated Time of Arrival: December 31
50 μL	$252	在庫あり	Estimated Time of Arrival: December 31
100 μL	$408	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

CD369 Antibody (YA4466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD369.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 28 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BXN2

Gene ID

64581 [NCBI]

Immunogen

Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

純度	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL FC ICC

Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD369 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Immunofluorescence analysis of Hela cells labeling CD369 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CD369 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L ( green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

Function：Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed:11567029, PubMed:12423684). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (By similarity). Enhances cytokine production in macrophages and dendritic cells (By similarity). Mediates production of reactive oxygen species in the cell (By similarity). Mediates phagocytosis of C.albicans conidia (PubMed:17230442). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans (By similarity)

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Cytoplasm; Cytoplasm; Cytoplasm

Expression：
Tissue_specificity:Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta

Induction:Up-regulated during differentiation from monocytes into dendritic cells

Isoforms & Post-Translational Modification：Q9BXN2 has 10 isomers: Q9BXN2-1: 27627 Da (predicted); Q9BXN2-2: 22563 Da (predicted); Q9BXN2-3: 21065 Da (predicted); Q9BXN2-4: 21130 Da (predicted); Q9BXN2-5: 19218 Da (predicted); Q9BXN2-6: 14787 Da (predicted); Q9BXN2-7: 16000 Da (predicted); Q9BXN2-8: 5164 Da (predicted); Q9BXN2-9: 8363 Da (predicted); Q9BXN2-10: 13138 Da (predicted).
Phosphorylated on tyrosine residues in response to beta-glucan binding

Subunit：Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens. Interacts with CD37; this interaction controls CLEC7A-mediated IL-6 production (PubMed:17182550)

Synonyms

CLEC7A; BGR; CANDF4; SCARE2; DECTIN1; CLECSF12

ドキュメンテーション

Select Batch:

データシート (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/hcc827-cell.html [Content Brief]