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MAP3K14 Antibody (YA4447)

(Synonyms: HS; NIK; HSNIK; FTDCR1B)
Customer Review

Based on 1 Customer Validation

MAP3K14 Antibody (YA4447) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP3K14.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • MAP3K14 Antibody (YA4447) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MAP3K14 Antibody (YA4447) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP3K14.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 104 kDa
Immunogen

Purified recombinant fragment of human MAP3K14 (AA: 769-947) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    Flow cytometric analysis of 1X10^6 Hela cells labeling MAP3K14 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MAP3K14 is a Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway. Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response. Could act in a receptor-selective manner[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon

  • Subunit

    Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14. Directly interacts with DDX3X (PubMed:30341167). Interacts (via C-terminus and kinase domain) with PPPC3A (via N-terminus) and PPP3CB (By similarity)

  • SwissProt ID

    Q99558

  • Gene ID
    9020 [NCBI]
  • Synonyms

    HS; NIK; HSNIK; FTDCR1B

MAP3K14 Antibody (YA4447) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling MAP3K14 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MAP3K14 Antibody (YA4447) Product Image
    1/16
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • MAP3K14 Antibody (YA4447) Product Image
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling MAP3K14 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • Experimental Validation Results for MAP3K14 Antibody (YA4447)
  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using HMAP3K14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84750, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • MAP3K14 Antibody (YA4447) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling MAP3K14 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

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  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image IHC-P
  • MAP3K14 Antibody (YA4447) Product Image FC