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Mucin 5AC Antibody (YA5673)

(Synonyms: Mucin-5AC; Gastric mucin; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin; MUC5; MUC5AC)
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Based on 1 Customer Validation

Mucin 5AC Antibody (YA5673) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Mucin 5AC.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
  • Mucin 5AC Antibody (YA5673) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-400 1:50-200 1:500-5000

Product Details

Description

Mucin 5AC Antibody (YA5673) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Mucin 5AC.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Calculated Molecular Weight Predicted band size: 527 kDa;
Immunogen

Synthesized peptide derived from human protein.AA range:2700-2900

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719065

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
    Immunocytochemistry analysis of HepG2 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
    Immunocytochemistry analysis of HT-29 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Mucin 5AC is a Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD)[1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is highly expressed in respiratory tract and gastric mucosal surface cells. It is overexpressed in various cancers. It is also expressed in Barrett's esophageal epithelium and proximal duodenum.

    Induction:By IL4

  • Subunit

    Homomultimer; disulfide-linked (PubMed:14718370). The N- and C-terminus mediate their assembly into higher order structures to form filaments (By similarity). The CTCK domains of two polypeptides associate in the endoplasmic reticulum to generate intermolecularly disulfide-bonded dimers (By similarity). These dimers progress to the Golgi apparatus, which is a more acidic environment than the endoplasmic reticulum. Under acidic conditions, the N-termini form non-covalent intermolecular interactions that juxtapose assemblies from different CTCK-linked dimers to produce long, disulfide-linked polymers that remain highly compact until secretion (By similarity)

  • SwissProt ID

    P98088

  • Gene ID
    4586 [NCBI]
  • Synonyms

    Mucin-5AC; Gastric mucin; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin; MUC5; MUC5AC

Mucin 5AC Antibody (YA5673) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HT-29 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Mucin 5AC Antibody (YA5673) Product Image
    1/16
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
  • Mucin 5AC Antibody (YA5673) Product Image
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HT-29 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
  • Experimental Validation Results for Mucin 5AC Antibody (YA5673)
  • Mucin 5AC Antibody (YA5673) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Mucin 5AC Antibody (YA5673) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HT-29 cells labeling Mucin 5AC with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mucin 5AC Antibody (HY-P85981) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Mucin 5AC Antibody (YA5673) Product Image ICC
  • Mucin 5AC Antibody (YA5673) Product Image ICC