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PODXL Antibody (YA3494)

(Synonyms: PC; PDX; PCLP; Gp200; gp135; PCLP-1; PODXL1)
Customer Review

Based on 1 Customer Validation

PODXL Antibody (YA3494) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PODXL.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • PODXL Antibody (YA3494) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

PODXL Antibody (YA3494) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PODXL.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 165 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Immunogen

Purified recombinant fragment of human PODXL (AA: Extra(23-172)) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718769

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PODXL Antibody (YA3494)
    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    Immunohistochemical analysis of paraffin-embedded human glioma tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PODXL is involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells

  • Subcellular Localization

    Apical cell membrane; Cell projection, lamellipodium; Cell projection, filopodium; Cell projection, ruffle; Cell projection, microvillus; Membrane raft; Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Glomerular epithelial cells (podocytes)

  • Isoforms & Post-Translational Modification

    O00592 has 2 isomers: O00592-1: 58635 Da (predicted); O00592-2: 55386 Da (predicted).
    N- and O-linked glycosylated. Sialoglycoprotein (By similarity)

  • Subunit

    Monomer; when associated with the membrane raft. Oligomer; when integrated in the apical membrane. Interacts (via the C-terminal PDZ-binding motif DTHL) with NHERF1 (via the PDZ domains); the interaction is not detected in glomerular epithelium cells, take place early in the secretory pathway and is necessary for its apical membrane sorting. Found in a complex with EZR, PODXL and NHERF2. Associates with the actin cytoskeleton through complex formation with EZR and NHERF2. Interacts (via the C-terminal PDZ-binding motif DTHL) with NHERF2 (via the PDZ 1 domain); interaction is detected in glomerular epithelium cells (By similarity). Interacts with EZR

  • SwissProt ID

    O00592

  • Gene ID
    5420 [NCBI]
  • Synonyms

    PC; PDX; PCLP; Gp200; gp135; PCLP-1; PODXL1

PODXL Antibody (YA3494) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image
    1/16
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • PODXL Antibody (YA3494) Product Image
  • Experimental Validation Results for PODXL Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • Experimental Validation Results for PODXL Antibody (YA3494)
  • PODXL Antibody (YA3494) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human glioma tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PODXL Antibody (HY-P83797, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PODXL Antibody (YA3494) Product Image IHC-P
  • PODXL Antibody (YA3494) Product Image IHC-P
  • PODXL Antibody (YA3494) Product Image IHC-P
  • PODXL Antibody (YA3494) Product Image IHC-P
  • PODXL Antibody (YA3494) Product Image IHC-P
  • PODXL Antibody (YA3494) Product Image IHC-P