CD133 Antibody (YA3538)

Art. -Nr.: HY-P83841: Data Sheet User Guide for Antibodies
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CD133 Antibody (YA3538) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD133.

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Größe	Verfügbarkeit	Menge
20 μL	Angebot einholen 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Angebot einholen 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Angebot einholen 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

CD133 Antibody (YA3538) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD133.

Host

Mouse

Clonality

Monoclonal Antibody

Molekulargewicht

Predicted band size: 97 kDa;

Observed band size: 133 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O43490

Gene ID

8842 [NCBI]

Immunogen

Purified recombinant fragment of human PROM1 (AA: 20-108) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439)

Subcellular Localization：Apical cell membrane; Multi-pass membrane protein; Cell projection, microvillus membrane; Multi-pass membrane protein; Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum; Endoplasmic reticulum-Golgi intermediate compartment

Expression：
Tissue_specificity:Isomer 1 is selectively expressed on CD34+ hematopoietic stem cells and progenitor cells in human bone marrow, fetal liver, umbilical cord blood, and human peripheral blood. Isomer 1 is not detected in other blood cells. Isomer 1 is also expressed in a variety of non-lymphoid tissues, including the retina, pancreas, placenta, kidney, liver, lung, brain, and heart. In saliva, isomer 1 exists as small membrane granules. Isomer 2 is primarily expressed in fetal liver, skeletal muscle, kidney, and heart, as well as in human pancreas, kidney, liver, lung, and placenta. Isomer 2 is highly expressed in fetal liver, lowly expressed in bone marrow, and almost undetectable in peripheral blood. Isomer 2 is expressed on hematopoietic stem cells and epidermal basal cells (protein level) . In the human retina, isomer 2 is expressed by rod cells and cone cells (protein level) .

Isoforms & Post-Translational Modification：O43490 has 7 isomers: O43490-1: 97202 Da (predicted); O43490-2: 96251 Da (predicted); O43490-3: 93270 Da (predicted); O43490-4: 93654 Da (predicted); O43490-5: 92741 Da (predicted); O43490-6: 93692 Da (predicted); O43490-7: 94605 Da (predicted).
Isoform 1 and isoform 2 are glycosylated;Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis

Subunit：Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B

Synonyms

RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

Dokumentation

User Guide for Antibodies (1077 KB)