Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449)

Cat. No.: HY-P83712M: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449) is a rat-derived and fluorescein isothiocyanate (FITC) IgG2b monoclonal antibody, targeting to CD16/32.

For research use only. We do not sell to patients.

Size	Stock	Quantity
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449) is a rat-derived and fluorescein isothiocyanate (FITC) IgG2b monoclonal antibody, targeting to CD16/32.

Host

Rat

Clonality

Monoclonal

Molecular Weight

Predicted band size: 29 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

P08508 / P08101

Gene ID

14130 [NCBI] / 14131 [NCBI]

Immunogen

A synthesized peptide derived from Mouse CD16/32

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	5μL per test for million cells or whole blood in 100 μL volume.

Conjugation	FITC	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% Proclin300, 1% BSA.

Storage & Stability

Stored at 2-8°C for 1 year, protect from light, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：P08508: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b (PubMed:17558411). Mediates neutrophil activation by IgG complexes redundantly with Fcgr4 (PubMed:18097064)
P08101: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR) , T-cell receptors for AG (TCR) , and Fc receptors, respectively

Subcellular Localization：P08508: Cell membrane; Single-pass type I membrane protein
P08101: Cell membrane; Single-pass type I membrane protein; Cytoplasm, cytoskeleton; Secreted

Expression：
Tissue_specificity: P08101: Widely expressed by cells of hemopoietic origin. The isoforms are differentially expressed. Isoform IIB1 is preferentially expressed by cells of the lymphoid lineage, isoform IIB2 by cells of the myeloid lineage, and isoform IIB3 is released by macrophages and is present in the serum. Isoform IIB1' is expressed in myeloid and lymphoid cell lines, in normal spleen cells, and in resting or LPS-activated B-cells but is not detected in mesenteric lymph node cells

Subunit：P08508: Interacts with INPP5D/SHIP1
P08101: Interacts with FGR (By similarity). Interacts with LYN

Database

Entrez Gene: 14130 Mouse ; 14131 Mouse

SwissProt: P08508 Mouse ; P08101 Mouse

Synonyms

CD16a/b; CD32; CD32A/B; Fc fragment of IgG low affinity IIa/b receptor; Fc fragment of IgG low affinity IIIa/b receptor; Fc fragment of IgG low affinity IIIb receptor; Fc gamma receptor III A/B; FCG2A; FcGR; FCGR2A/BFCGR3; FCGR3A/B; Fc gamma RIIa/b

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449)

Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449) Featured Recommendations:

Product Detail

Background

Documentation

Rat Anti-Mouse CD16/32 Antibody (FITC)(YA3449) Related Classifications

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