TIE1 Antibody (YA3973)

Cat. No.: HY-P84276: Data Sheet COA
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TIE1 Antibody (YA3973) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIE1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

TIE1 Antibody (YA3973) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIE1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 125 kDa;

Observed band size: 125 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P35590

Gene ID

7075 [NCBI]

Immunogen

Purified recombinant fragment of human TIE1 (AA: 385-607) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HL7702(lane 1(40μg) ),BEL-7402(lane 2(40μg) ),SMMC-7721(lane 3(40μg) )and HT1080(lane 4(40μg) ) using TIE1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Specifically expressed in developing vascular endothelial cells

Isoforms & Post-Translational Modification：P35590 has 3 isomers: P35590-1: 125090 Da (predicted); P35590-2: 40616 Da (predicted); P35590-3: 34138 Da (predicted).
Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2

Subunit：Heterodimer with TEK/TIE2 (PubMed:20227369). Interacts with SVEP1 (via C-terminus) (PubMed:37097004)

Synonyms

TIE; JTK14

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/thp-1-cell.html [Content Brief]