Art. -Nr.: HY-114252

Strophanthidin

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Strophanthidin is a naturally available cardiac glycoside. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal. Strophanthidin increases both diastolic and systolic intracellular Ca²⁺ concentration.

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Reinheit: 98.71%
CAS. Nr.: 66-28-4
Formel: C₂₃H₃₂O₆
Molecular Weight:404.50

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Strophanthidin Chemical Structure

CAS. Nr.: 66-28-4

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Strophanthidin is a naturally available cardiac glycoside^[1]. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal^[2]. Strophanthidin increases both diastolic and systolic intracellular Ca²⁺ concentration^[3].

IC₅₀ & Target

Na+/K+-ATPase^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
1A9	ED₅₀	0.15 μg/mL Compound: 19	Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
A549	ED₅₀	0.11 μg/mL Compound: 19	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
CAKI-1	ED₅₀	0.14 μg/mL Compound: 19	Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
KB	ED₅₀	0.44 μg/mL Compound: strophanthidin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 4031899]
KB	ED₅₀	0.61 μg/mL Compound: 19	Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
MCF7	ED₅₀	0.38 μg/mL Compound: 19	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
PC-3	ED₅₀	0.17 μg/mL Compound: 19	Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
SK-MEL-2	ED₅₀	0.13 μg/mL Compound: 19	Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
U-251	EC₅₀	2 μM Compound: 10	Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay	[PMID: 19405508]
U-87MG ATCC	ED₅₀	0.72 μg/mL Compound: 19	Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]

In Vitro

Strophanthidin (0~10 μM; 24 hours; MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells^[1].
Strophanthidin (0.5 to 500 μM; PBMCs) does not show significant cytotoxicity in PBMCs. Strophanthidin (2 μM; MCF-7 cells) can arrest cell cycle at the G2/M phase^[1].
Strophanthidin (MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells. Strophanthidin (MCF-7, A549, and HepG2 cells) inhibits the expression of checkpoint and cyclin-dependent kinases in three cancer cells compared to untreated controls. Strophanthidin can modulate the protein localization from the nucleus to the membrane as well as to the cytoplasm. Strophanthidin is a monosaccharide cardiac glycoside with one aglycone portion and without any sugar unit. Strophanthidin induces apoptosis by the attenuation of multiple biochemical signaling pathways and by arresting cell cycle at the G2/M phase through p53-dependent and p53-independent mechanisms^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MCF-7, A549, and HepG2 cells
Concentration:

0~10 μM
Incubation Time:

24 hours
Result:

Inhibited the proliferation in three different cancer cells.

Chemical Information

CAS. Nr. 66-28-4
Appearance Solid
Molecular Weight 404.50
Formel C₂₃H₃₂O₆
Color White to off-white
SMILES

O=C[C@@]12[C@]3([H])[C@](CC[C@@]1(C[C@@H](O)CC2)O)([H])[C@@]4([C@@](C)([C@@H](C5=CC(OC5)=O)CC4)CC3)O
Structure Classification
- Steroids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (2)

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Int J Surg

A multi-dimensional bioinformatic dissection of the molecular mechanisms in high BMI-associated colorectal cancer: identification and validation of EGLN1 as a key target. [Abstract]

2025 Nov 5. PMID: 41191606
bioRxiv

2026 Jun 24.

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 25 mg/mL (61.80 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4722 mL

12.3609 mL

24.7219 mL

5 mM

0.4944 mL

2.4722 mL

4.9444 mL

10 mM

0.2472 mL

1.2361 mL

2.4722 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Reddy D, et al. Strophanthidin Attenuates MAPK, PI3K/AKT/mTOR, and Wnt/β-Catenin Signaling Pathways in Human Cancers. Front Oncol. 2020;9:1469. Published 2020 Jan 17. [Content Brief]

[2]. Su SW, et al. Relationship between cardiotonic effects and inhibition on cardiac sarcolemmal Na+,K+-ATPase of strophan-thidin at low concentrations. Acta Pharmacol Sin. 2003;24(11):1103-1107. [Content Brief]

[3]. Bennett DL, et al. Strophanthidin-induced gain of Ca2+ occurs during diastole and not systole in guinea-pig ventricular myocytes. Pflugers Arch. 1999;437(5):731-736. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	2.4722 mL	12.3609 mL	24.7219 mL	61.8047 mL
	5 mM	0.4944 mL	2.4722 mL	4.9444 mL	12.3609 mL
	10 mM	0.2472 mL	1.2361 mL	2.4722 mL	6.1805 mL
	15 mM	0.1648 mL	0.8241 mL	1.6481 mL	4.1203 mL
	20 mM	0.1236 mL	0.6180 mL	1.2361 mL	3.0902 mL
	25 mM	0.0989 mL	0.4944 mL	0.9889 mL	2.4722 mL
	30 mM	0.0824 mL	0.4120 mL	0.8241 mL	2.0602 mL
	40 mM	0.0618 mL	0.3090 mL	0.6180 mL	1.5451 mL
	50 mM	0.0494 mL	0.2472 mL	0.4944 mL	1.2361 mL
	60 mM	0.0412 mL	0.2060 mL	0.4120 mL	1.0301 mL

Keywords

Strophanthidin

2 Cited Publications

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Strophanthidin

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Publications Citing Use of MedChemExpress (MCE) Strophanthidin

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Strophanthidin

Membrane Transporter/Ion Channel Compound Library

Natural Product Library

Lipid Compound Library

Anti-Cardiovascular Disease Compound Library

Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Membrane Protein-targeted Compound Library

Cysteine Targeted Covalent Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Tibetan Medicine Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Strophanthidin

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Strophanthidin Related Classifications

HY-114252 Related Classifications