ATTO 647 NHS ester 

ATTO 647 NHS ester is a fluorescent label targeting free amino groups. ATTO 647 NHS ester can undergo a nucleophilic reaction with the free amino groups of EF-C peptide via its activated carboxylic acid group to form a stable covalent conjugate. ATTO 647 NHS ester-labeled nanofibers not only retain retroviral transduction-enhancing activity but also maintain stable fluorescent signals in both buffer and cell culture systems. ATTO 647 NHS ester is suitable for detection applications including fluorescence spectroscopy, microscopy and flow cytometry. ATTO 647 NHS ester has been used in studies related to HIV-1 infection^[1][2].

ATTO 647 NHS ester (2-fold molar excess; 1 h) covalently labels EF-C peptide at free amino groups, and mixing labeled peptide with a 100-fold to 1000-fold excess of unlabeled EF-C peptide yields fluorescent, morphologically intact EF-C PNF with peak emission at 674 nm^[2].
ATTO 647 NHS ester (0.2-25 μg/mL; 3 days)-labeled EF-C PNF are not cytotoxic to TZM-bl cells at concentrations up to 25 μg/mL after 3 days of incubation^[2].
ATTO 647 NHS ester (100 μg/mL; 250 min)-labeled EF-C PNF maintain stable fluorescence intensity in PBS over 250 min, outperforming non-covalent amyloid probes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[ATTO 647 NHS ester]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. IVANOVA S, GABROVSKA K, GRIGOROVA G, et al. Evaluation of the efficiency of three different fluorescent conjugates used as tracersin penicillin magnetic nanoparticle immunoassay[C]//First National Conference of Biotechnology, Sofia. 2015, 100: 164-176.

  [2]. Rode S, et al. Generation and Characterization of Virus-Enhancing Peptide Nanofibrils Functionalized with Fluorescent Labels. Bioconjug Chem. 2017;28(4):1260-1270.