ATTO 647 NHS ester
ATTO 647 NHS ester is a fluorescent label targeting free amino groups. ATTO 647 NHS ester can undergo a nucleophilic reaction with the free amino groups of EF-C peptide via its activated carboxylic acid group to form a stable covalent conjugate. ATTO 647 NHS ester-labeled nanofibers not only retain retroviral transduction-enhancing activity but also maintain stable fluorescent signals in both buffer and cell culture systems. ATTO 647 NHS ester is suitable for detection applications including fluorescence spectroscopy, microscopy and flow cytometry. ATTO 647 NHS ester has been used in studies related to HIV-1 infection.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
ATTO 647 NHS ester (2-fold molar excess; 1 h) covalently labels EF-C peptide at free amino groups, and mixing labeled peptide with a 100-fold to 1000-fold excess of unlabeled EF-C peptide yields fluorescent, morphologically intact EF-C PNF with peak emission at 674 nm[2].
ATTO 647 NHS ester (0.2-25 μg/mL; 3 days)-labeled EF-C PNF are not cytotoxic to TZM-bl cells at concentrations up to 25 μg/mL after 3 days of incubation[2].
ATTO 647 NHS ester (100 μg/mL; 250 min)-labeled EF-C PNF maintain stable fluorescence intensity in PBS over 250 min, outperforming non-covalent amyloid probes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:TZM-bl cells
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Concentration:0.2, 1, 5, 25 μg/mL
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Incubation Time:3 days
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Result:Showed no reduction in TZM-bl cell viability relative to unlabeled EF-C PNF and controls at all tested concentrations.
Chemical Information
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SMILES
[ATTO 647 NHS ester]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ATTO 647 NHS ester Related Classifications
HY-D1993 Related Classifications
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