Aβ aggregation modulator-1

Organotypic brain slice culture is an ex vivo method in which CNS tissue slices are maintained on a stable support with culture medium and oxygen access, preserving tissue architecture, multiple resident brain cell types, and network organization better than dissociated cultures. The commonly used membrane-interface method places brain or hippocampal slices on a porous membrane insert at the air-liquid interface; culture medium reaches the tissue through the membrane while the slice remains oxygenated from the humidified incubator atmosphere. Readouts depend on the experimental aim: slice survival can be monitored by propidium iodide uptake or LDH release, tissue organization by immunostaining, live structural changes by repeated imaging, and neuronal/network function by electrophysiology or multi-electrode recordings.

Congo red amyloid staining is a histochemical method used to detect extracellular amyloid deposits in tissue sections based on the affinity of Congo red dye for β-pleated sheet-rich protein aggregates. When bound to amyloid, Congo red produces characteristic apple-green birefringence under polarized light microscopy, which is widely regarded as a diagnostic feature of amyloid deposition in histopathology. The diagnostic principle relies on the combination of dye binding (congophilia) and optical anisotropy under polarized illumination, which distinguishes amyloid from most non-amyloid eosinophilic extracellular deposits in routine histological evaluation. Amyloid identification by Congo red staining remains a cornerstone in diagnostic pathology despite the availability of adjunct methods such as immunohistochemistry and mass spectrometry, particularly because of its ability to localize deposits directly within tissue architecture. The specificity of Congo red-positive deposits is incre

Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.