BETA-1 

BETA-1 is the first twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule. BETA-1 emits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria. BETA-1 can be used for the simultaneous and dual-color imaging of LDs and mitochondria in vivo and in vitro^[1].

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
Wavelength: Blue: λex = 405 nm, λem = 430 - 500 nm; Red: λex = 561 nm, λem = 600 - 700 nm.
The HeLa cells were cultured in DMEM medium supplemented with 10% FBS, in a 37 °C CO?/air (5%/95%) incubator.
2. 5.0 × 103 HeLa cells were inoculated into each well of a 96-well glass-bottom plate and incubated for 24 hours.
3. For cell imaging, live HeLa cells were stained with 5 μM BETA-1 at 37 °C for 30 minutes.
4. Collect the emission light of BETA-1 under the excitation wavelengths of 405 or 561 nm. Record digital images using a laser scanning confocal microscope. Analyze the images using Fiji/ImageJ.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

705.67

C₃₇H₂₈IN₃S₂

2924598-24-1

Solid

Brown to black

C[N+]1=C(/C=C/C2=CC=C(N(C3=CC=C(/C=C/C4=NC5=CC=CC=C5S4)C=C3)C6=CC=CC=C6)C=C2)SC7=CC=CC=C17.[I-]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Zeng ST, et al. Construction of a TICT-AIE-Integrated Unimolecular Platform for Imaging Lipid Droplet-Mitochondrion Interactions in Live Cells and In Vivo. ACS Sens. 2022 Dec 19.  [Content Brief]