BMS-1166 hydrochloride
Based on 6 publication(s) in Google Scholar
BMS-1166 hydrochloride is a potent PD-1/PD-L1 immune checkpoint inhibitor. BMS-1166 hydrochloride induces dimerization of PD-L1 and blocks its interaction with PD-1, with an IC50 of 1.4 nM. BMS-1166 hydrochloride antagonizes the inhibitory effect of PD-1/PD-L1 immune checkpoint on T cell activation.
For research use only. We do not sell to patients.
- Purity: 99.31%
- CAS No.: 2113650-05-6
- Formula: C36H34Cl2N2O7
- Molecular Weight:677.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Publications Citing Use of MedChemExpress (MCE) BMS-1166 hydrochloride
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Biological Activity
IC50: 1.4 nM (PD-1/PD-L1 interaction)[1].
BMS-1166 is a potent PD-1/PD-L1 interaction inhibitor with an IC50 of 1.4 nM in a homogenous time-resolved fluorescence binding assay[1]. BMS-1166 antagonizes the inhibitory effect of PD-1/PD-L1 immune checkpoint on T cell activation. BMS-1166 dose dependently abolishes the inhibition of ECs stimulation by sPD-L1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2113650-05-6
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Appearance Solid
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Molecular Weight 677.57
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Formula C36H34Cl2N2O7
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Color White to off-white
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SMILES
O=C(O)[C@@H]1N(CC2=CC(Cl)=C(OCC3=CC=CC(C4=CC=C(OCCO5)C5=C4)=C3C)C=C2OCC6=CC=CC(C#N)=C6)C[C@H](O)C1.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Cells
Suppressing PD-L1 Expression via AURKA Kinase Inhibition Enhances Natural Killer Cell-Mediated Cytotoxicity against Glioblastoma. [Abstract]2024 Jul 6;13(13):1155. PMID: 38995006 -
Chem Biol Interact
Paris saponin VII restrains PD-L1 mediated immune evasion through the AKT1 and STAT3 signaling pathways. [Abstract]2025 May 17:111562. PMID: 40389195 -
Int J Med Sci
Dual PI3K/mTOR Inhibitor BEZ235 combined with BMS-1166 Promoting Apoptosis in Colorectal Cancer. [Abstract]2024 Jul 9;21(10):1814-1823. PMID: 39113885
BMS-1166 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Med Sci. 2024 Jul 9;21(10):1814-1823. [Abstract]
Cell viability of SW480 and SW480R cells treated with BMS-1166 (0.2, 0.5, 1, 2 μM) after 24 h was assessed by MTT assays.
BMS-1166 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Med Sci. 2024 Jul 9;21(10):1814-1823. [Abstract]
Colony formation assays were used to determine the clonogenic capacity of SW480 and SW480R cells treated with BMS-1166 (0.5 μM).
BMS-1166 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Med Sci. 2024 Jul 9;21(10):1814-1823. [Abstract]
Expression and phosphorylation of S6K and elF4EBP1 after treatment with BEZ235 plus BMS-1166 (0.5 μM) in SW480 and SW480R cells.
BMS-1166 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Med Sci. 2024 Jul 9;21(10):1814-1823. [Abstract]
Wound healing assays were carried out to assess the migration ability of CRC cells treated with BMS-1166, BEZ235, or the combination of the two drugs for 12, 24, and 48 h.
BMS-1166 hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Med Sci. 2024 Jul 9;21(10):1814-1823. [Abstract]
CRC cells were treated with BMS-1166 for 24 h, stained with JC-1, and detected by flow cytometry.
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Biochem Biophys Res Commun
Baicalin inhibits bladder cancer progression by suppressing PD-L1-mediated M2 macrophage polarization via ALDH2. [Abstract]2025 Nov 21:794:153036. PMID: 41330073 -
Bio Protoc
Establishment of Human PD-1/PD-L1 Blockade Assay Based on Surface Plasmon Resonance (SPR) Biosensor. [Abstract]2023 Aug 5;13(15):e4765. PMID: 37575393 -
J Vis Exp
2025 May 2:(219). PMID: 40388341
Solvent & Solubility
DMSO : ≥ 100 mg/mL (147.59 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
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- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Guzik K, et al. Small-Molecule Inhibitors of the Programmed Cell Death-1/Programmed Death-Ligand 1 (PD-1/PD-L1) Interaction via Transiently Induced Protein States and Dimerization of PD-L1. J Med Chem. 2017 Jul 13;60(13):5857-5867. [Content Brief]
[2]. Skalniak L, et al. Small-molecule inhibitors of PD-1/PD-L1 immune checkpoint alleviate the PD-L1-induced exhaustion of T-cells. Oncotarget. 2017 Aug 7;8(42):72167-72181. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4759 mL | 7.3793 mL | 14.7586 mL | 36.8966 mL |
| 5 mM | 0.2952 mL | 1.4759 mL | 2.9517 mL | 7.3793 mL | |
| 10 mM | 0.1476 mL | 0.7379 mL | 1.4759 mL | 3.6897 mL | |
| 15 mM | 0.0984 mL | 0.4920 mL | 0.9839 mL | 2.4598 mL | |
| 20 mM | 0.0738 mL | 0.3690 mL | 0.7379 mL | 1.8448 mL | |
| 25 mM | 0.0590 mL | 0.2952 mL | 0.5903 mL | 1.4759 mL | |
| 30 mM | 0.0492 mL | 0.2460 mL | 0.4920 mL | 1.2299 mL | |
| 40 mM | 0.0369 mL | 0.1845 mL | 0.3690 mL | 0.9224 mL | |
| 50 mM | 0.0295 mL | 0.1476 mL | 0.2952 mL | 0.7379 mL | |
| 60 mM | 0.0246 mL | 0.1230 mL | 0.2460 mL | 0.6149 mL | |
| 80 mM | 0.0184 mL | 0.0922 mL | 0.1845 mL | 0.4612 mL | |
| 100 mM | 0.0148 mL | 0.0738 mL | 0.1476 mL | 0.3690 mL |
BMS-1166 hydrochloride Related Classifications
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