Boc-Val-Pro-Arg-AMC
Based on 1 Customer Validation
Boc-Val-Pro-Arg-MCA is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.
For research use only. We do not sell to patients.
- Purity: 99.26%
- CAS No.: 65147-04-8
- Formula: C31H45N7O7
- Molecular Weight:627.73
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Trypsin activity assay[2]
1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0).
2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-MCA.
3. Incubate at 55 °C for 10 min.
4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction.
5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm).
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 65147-04-8
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Appearance Solid
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Molecular Weight 627.73
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Formula C31H45N7O7
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Color White to off-white
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Sequence
{Boc}-Val-Pro-Arg-{AMC}
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Sequence Shortening
{Boc}-VPR-{AMC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (159.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5930 mL | 7.9652 mL | 15.9304 mL | 39.8260 mL |
| 5 mM | 0.3186 mL | 1.5930 mL | 3.1861 mL | 7.9652 mL | |
| 10 mM | 0.1593 mL | 0.7965 mL | 1.5930 mL | 3.9826 mL | |
| 15 mM | 0.1062 mL | 0.5310 mL | 1.0620 mL | 2.6551 mL | |
| 20 mM | 0.0797 mL | 0.3983 mL | 0.7965 mL | 1.9913 mL | |
| 25 mM | 0.0637 mL | 0.3186 mL | 0.6372 mL | 1.5930 mL | |
| 30 mM | 0.0531 mL | 0.2655 mL | 0.5310 mL | 1.3275 mL | |
| 40 mM | 0.0398 mL | 0.1991 mL | 0.3983 mL | 0.9957 mL | |
| 50 mM | 0.0319 mL | 0.1593 mL | 0.3186 mL | 0.7965 mL | |
| 60 mM | 0.0266 mL | 0.1328 mL | 0.2655 mL | 0.6638 mL | |
| 80 mM | 0.0199 mL | 0.0996 mL | 0.1991 mL | 0.4978 mL | |
| 100 mM | 0.0159 mL | 0.0797 mL | 0.1593 mL | 0.3983 mL |
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