Boc-Val-Pro-Arg-AMC

Name: Boc-Val-Pro-Arg-AMC
Brand: MedChemExpress
SKU: HY-P4326
Rating: 4.5 (0 reviews)

Cat. No.: HY-P4326 Purity: 99.26%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Boc-Val-Pro-Arg-MCA is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Boc-Val-Pro-Arg-AMC Chemical Structure

CAS No. : 65147-04-8

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of Boc-Val-Pro-Arg-AMC:

Boc-Val-Pro-Arg-AMC hydrochloride In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Boc-Val-Pro-Arg-MCA is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity^[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Trypsin activity assay^[2]
1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0).
2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-MCA.
3. Incubate at 55 °C for 10 min.
4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction.
5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm). Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

627.73

Formula

C₃₁H₄₅N₇O₇

CAS No.

65147-04-8

Appearance

Solid

Color

White to off-white

Sequence

{Boc}-Val-Pro-Arg-{AMC}

Sequence Shortening

{Boc}-VPR-{AMC}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (159.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5930 mL	7.9652 mL	15.9304 mL
5 mM	0.3186 mL	1.5930 mL	3.1861 mL
10 mM	0.1593 mL	0.7965 mL	1.5930 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.26%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (251 KB) LCMS (94 KB) Elemental Analysis Report (110 KB)

Handling Instructions (2659 KB)

References

[1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204. [Content Brief]

[2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5930 mL	7.9652 mL	15.9304 mL	39.8260 mL
	5 mM	0.3186 mL	1.5930 mL	3.1861 mL	7.9652 mL
	10 mM	0.1593 mL	0.7965 mL	1.5930 mL	3.9826 mL
	15 mM	0.1062 mL	0.5310 mL	1.0620 mL	2.6551 mL
	20 mM	0.0797 mL	0.3983 mL	0.7965 mL	1.9913 mL
	25 mM	0.0637 mL	0.3186 mL	0.6372 mL	1.5930 mL
	30 mM	0.0531 mL	0.2655 mL	0.5310 mL	1.3275 mL
	40 mM	0.0398 mL	0.1991 mL	0.3983 mL	0.9957 mL
	50 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7965 mL
	60 mM	0.0266 mL	0.1328 mL	0.2655 mL	0.6638 mL
	80 mM	0.0199 mL	0.0996 mL	0.1991 mL	0.4978 mL
	100 mM	0.0159 mL	0.0797 mL	0.1593 mL	0.3983 mL