2. Buprofezin

Buprofezin is a broad-spectrum insecticide and chitin synthesis inhibitor that targets developmental stage coleopteran pests.Buprofezin promotes the conversion of energy metabolism from the aerobic tricarboxylic acid (TCA) cycle and oxidative phosphorylation to anaerobic glycolysis. Buprofezin also promotes the production of reactive oxygen species (ROS) by inhibiting cytochrome c oxidase.

For research use only. We do not sell to patients.

Buprofezin Chemical Structure

Buprofezin Chemical Structure

CAS No. : 69327-76-0

Size Price Stock Quantity
10 mg USD 25 In-stock
50 mg USD 42 In-stock
100 mg USD 70 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of Buprofezin:

Buprofezin Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • Customer Review

Description

Buprofezin is a broad-spectrum insecticide and chitin synthesis inhibitor that targets developmental stage coleopteran pests.Buprofezin promotes the conversion of energy metabolism from the aerobic tricarboxylic acid (TCA) cycle and oxidative phosphorylation to anaerobic glycolysis. Buprofezin also promotes the production of reactive oxygen species (ROS) by inhibiting cytochrome c oxidase[1][2].

In Vitro

Buprofezin (100, 300, 1000 µM) significantly reduces viability of HepG2 cells[1].
Buprofezin (3, 10, 30 µM; 24 h) promotes the conversion of energy metabolism from the aerobic tricarboxylic acid (TCA) cycle and oxidative phosphorylation to anaerobic glycolysis in HepG2 cells[1].
Buprofezin (3, 10, 30 µM; 24 h) inhibits the activity of Complex IV in HepG2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Buprofezin Related Antibodies

RT-PCR[1]

Cell Line: HepG2 cells
Concentration: 3, 10, 30 µM
Incubation Time: 24 h
Result: Significantly increased lactate dehydrogenase B (LDHB) levels when at 30 µM, and slightly increased 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3).
Decreased ATP levels in a concentration-dependent manner to 91.3, 87.9 and 67.2% of the levels in the vehicle control under treatment with 3, 10 and 30 µM buprofezin, respectively.
Significantly increased the lactate levels.

Immunofluorescence[1]

Cell Line: HepG2 cells
Concentration: 3, 10, 30 µM
Incubation Time: 24 h
Result: Significantly inhibited the activity of Complex IV to 82.2, 69.2 and 63.4% of the vehicle control levels following buprofezin treatment at 3, 10 and 30 µM, respectively.

Cell Viability Assay[1]

Cell Line: HepG2 cells
Concentration: 3, 10, 30 µM
Incubation Time: 24 h
Result: Significantly increased the intracellular ROS levels in a concentrate-independent manner, and decreased mtDNA contents.
In Vivo

Buprofezin (46.3, 139, 417 mg/kg; p.o.; single) accumulates in the liver (35.84%), brain (23.58%), stomach (21.94%) and kidney (18.64%)[1].
Buprofezin (46.3, 139, 417 mg/kg; p.o.; single) elevates the MDA level in all organs (especially in the liver and brain) in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (6 to 8-week-old)[1].
Dosage: 46.3, 139, 417 mg/kg
Administration: Oral administration; single
Result: Tended to elevate the MDA level in all organs, and the most significant concentration-dependent increases were observed in the liver and brain.
Exhibited the highest concentrations in the liver (35.84%) followed by the brain (23.58%), stomach (21.94%) and kidney (18.64%), while the levels in the mouse spleen and heart were below the limit of detection.
Molecular Weight

305.44

Formula

C16H23N3OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(C(C)C)/C(SCN1C2=CC=CC=C2)=N/C(C)(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (327.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2740 mL 16.3698 mL 32.7397 mL
5 mM 0.6548 mL 3.2740 mL 6.5479 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.55%

COA (252 KB) HNMR (243 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2740 mL 16.3698 mL 32.7397 mL 81.8491 mL
5 mM 0.6548 mL 3.2740 mL 6.5479 mL 16.3698 mL
10 mM 0.3274 mL 1.6370 mL 3.2740 mL 8.1849 mL
15 mM 0.2183 mL 1.0913 mL 2.1826 mL 5.4566 mL
20 mM 0.1637 mL 0.8185 mL 1.6370 mL 4.0925 mL
25 mM 0.1310 mL 0.6548 mL 1.3096 mL 3.2740 mL
30 mM 0.1091 mL 0.5457 mL 1.0913 mL 2.7283 mL
40 mM 0.0818 mL 0.4092 mL 0.8185 mL 2.0462 mL
50 mM 0.0655 mL 0.3274 mL 0.6548 mL 1.6370 mL
60 mM 0.0546 mL 0.2728 mL 0.5457 mL 1.3642 mL
80 mM 0.0409 mL 0.2046 mL 0.4092 mL 1.0231 mL
100 mM 0.0327 mL 0.1637 mL 0.3274 mL 0.8185 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Buprofezin69327-76-0Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Buprofezin
Cat. No.:
HY-B0831
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.